Name: Anti-FANCI (Ser559) Antibody
Brand: PhosphoSolutions
SKU: p171-559
Price: 415.0 USD
Availability: InStock

Our Anti-FANCI (Ser559) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human FANCI (Ser559) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

FANCI

Molecular Weight

150 kDa

Go to Full Product Details

Western blot of HeLa cell lysates that had been treated with UV (~254 nm) for 0’ or 20’ showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559.

Western blot of Hela cell lysate treated with UV (~254nm) for 20’ showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

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$415

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Product Details

Target

FANCI (Ser559)

Target Description

Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al., 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitnation by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation of Ser-556 and Ser-559 are highly conserved and have been determined to be ATM/ATR kinase substrates for the FA-DNA pathway (Smogorzewska et al., 2007). Furthermore, the phosphorylation of Ser-556 and Ser-559 have been shown to play key roles in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014). Research continues to determine if one site plays more of a role in the DNA repair/damage checkpoint of the FA pathway and stabilization of the ID2 complex.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

FANCI

Molecular Weight

150 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser559 of human FANCI, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human

Expected Reactivity

Non-Human Primate

Antibody Registry ID

AB_2636808

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:250

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~150 kDa FANCI protein phosphorylated at Ser559. Immunolabeling is increased when HeLa cells are treated with 256nm UV light for 20 minutes and completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser559

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

FANCI antibody, FANCI gene antibody, FANCI_HUMAN antibody, Fanconi anemia group I protein antibody, Fanconi anemia complementation group I antibody, FLJ10719 antibody, FLJ14658 antibody, KIAA1794 antibody, Protein FACI antibody, Protein FANCI antibody,

UniProt Details

UniProt (Human): Q9NVI1
UniProt (Immunogen Species): Q9NVI1

NCBI Gene ID

55215

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
28636932	not listed	Cheung, R., et al. 2017. Ubiquitination-linked phosphorylation of the FANCI S/TQ cluster contributes to activation of the Fanconi anemia I/D2 complex. Cell Reports, 19(12), pp.2432-2440.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-FANCI (Ser559) Antibody

Our Anti-FANCI (Ser559) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human FANCI (Ser559) and is antigen affinity purified from pooled serum. It is great for use in WB.