Anti-FAK (Tyr-397), Phosphospecific Antibody (M121)

Name: Anti-FAK (Tyr-397), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: FM1211
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M121

Applications

Host Species

Mouse

Format

Protein A Purified

Gene Name

PTK2

Molecular Weight

125

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Western blot analysis of HUVECs untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FAK (lanes 1 & 2) and anti-FAK (Tyr-397) (lanes 3 & 4).

Volume: 100 µL

Variant

Price:

$309

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Bulk Order Anti-FAK (Tyr-397), Phosphospecific Antibody

Product Details

Target

FAK (Tyr-397)

Target Description

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in signal transduction pathways important for cell spreading, migration and survival. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr-397, which is a binding site for Src family kinases, PI3-Kinase, and PLCγ. The recruitment of Src family kinases results in the phosphorylation of tyrosine 407, 576, and 577 in the catalytic domain, and tyrosine 871 and 925 in the carboxy-terminal region of FAK. Thus, the phosphorylation of Tyr-397 is a critical step in the activation of FAK.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M121

Isotype

IgG1

Applications

Host Species

Mouse

Gene Name

PTK2

Molecular Weight

125

Antigen

Clone (M121) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 397 of human FAK. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse FAK.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

The antibody detects a 125 kDa* protein on SDS-PAGE immunoblots of untreated HUVEC cells. This phosphorylated band is greatly reduced after treatment with alkaline phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-397

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PTK2

UniProt Details

UniProt (Human): Q05397
UniProt (Immunogen Species): Q05397

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Anti-FAK (Tyr-397), Phosphospecific Antibody (M121)

Anti-FAK (Tyr-397), Phosphospecific Antibody
SKU: FM1211

Bulk Order Anti-FAK (Tyr-397), Phosphospecific Antibody

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Anti-FAK (Tyr-397), Phosphospecific Antibody (M121)

Anti-FAK (Tyr-397), Phosphospecific Antibody SKU: FM1211

Bulk Order Anti-FAK (Tyr-397), Phosphospecific Antibody

Product Details

Anti-FAK (Tyr-397), Phosphospecific Antibody
SKU: FM1211