Name: Anti-EWS Antibody
Brand: PhosphoSolutions
SKU: 540-EWS
Price: 415.0 USD
Availability: InStock

Our Anti-EWS primary antibody from PhosphoSolutions is mouse monoclonal. It detects canine, human, mouse, and rat EWS and is Protein G purified. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Canine, Human, Mouse, Rat

Clone

5H7

Applications

ICC, IHC, WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

EWSR1

Molecular Weight

85 kDa

Go to Full Product Details

Immunofluorescence of HeLa cells showing clear localized labeling of the DNA in the nucleus with Anti-EWS (cat. 540-EWS, 1:1000, green) while Anti-Vimentin (cat. 2105-VIM, 1:5000, red) labels the cytoplasmic intermediate filament, and Hoechst DNA stain (blue).

Western blot of HeLa cell lysate showing specific immunolabeling of the ~85 kDa EWS protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-EWS Antibody

Product Details

Target

EWS

Target Description

The Ewing Sarcoma Breakpoint Region 1 (EWSR1) gene encodes the EWS protein, which along with FUS and TAF15 comprises the FET family of RNA-binding proteins. EWS is primarily expressed in the nucleus, but has also been found in the cytoplasm of secretory cell types (Andersson et al., 2008). Defects of EWSR1 are known to cause Ewing sarcoma and have been implicated in amyotrophic lateral sclerosis (ALS) (Svetoni et al., 2016). Recently, EWS has been shown to directly interact with oncogenic ETS transcription factors in the promotion of prostate cancer (Kedage et al., 2016).

Format

Protein G Purified

Clonality

Monoclonal

Clone

5H7

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

EWSR1

Molecular Weight

85 kDa

Antigen

Full length recombinant human EWS expressed in and purified from E. coli.

Species Reactivity

Canine, Human, Mouse, Rat

Antibody Registry ID

AB_2650521

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified from culture supernatant.

Buffer

PBS + 50% glycerol and 5 mm NaN3

Dilution Ranges

WB: 1:1000
IHC: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~85 kDa EWS protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

4 Ewing sarcoma breakpoint region 1 protein antibody, Ewing sarcoma breakpoint region 1 antibody, Ewing sarcoma breakpoint region 1 protein antibody, Ewings sarcoma EWS Fli1 type 1 oncogene antibody, EWS antibody, EWS oncogene antibody, EWS RNA binding protein 1 antibody, EWS_HUMAN antibody, EWSR 1 antibody, Ewsr1 antibody, EWSR1 protein antibody, RNA binding protein EWS antibody, RNA-binding protein EWS antibody

UniProt Details

UniProt (Human): Q01844
UniProt (Immunogen Species): Q01844

NCBI Gene ID

2130

Product Specific Protocols

Western Blot Protocol: Download

General References

Andersson, M.K., Ståhlberg, A., Arvidsson, Y., Olofsson, A., Semb, H., Stenman, G., Nilsson, O. and Åman, P., 2008. The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response. BMC Cell Biology, 9(1), p.37. PMID: 18620564
Svetoni, F., Frisone, P. and Paronetto, M.P., 2016. Role of FET proteins in neurodegenerative disorders. RNA Biology, 13(11), pp.1089-1102. PMID: 27415968
Kedage, V., Selvaraj, N., Nicholas, T.R., Budka, J.A., Plotnik, J.P., Jerde, T.J. and Hollenhorst, P.C., 2016. An interaction with Ewing’s sarcoma breakpoint protein EWS defines a specific oncogenic mechanism of ETS factors rearranged in prostate cancer. Cell Reports, 17(5), pp.1289-1301. PMID: 27783944

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Anti-EWS Antibody (5H7)

Our Anti-EWS primary antibody from PhosphoSolutions is mouse monoclonal. It detects canine, human, mouse, and rat EWS and is Protein G purified. It is great for use in WB, IHC, ICC.

Anti-EWS Antibody
SKU: 540-EWS

Bulk Order Anti-EWS Antibody

Product Details

Product Specific Protocols

General References

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Anti-EWS Antibody (5H7)

Our Anti-EWS primary antibody from PhosphoSolutions is mouse monoclonal. It detects canine, human, mouse, and rat EWS and is Protein G purified. It is great for use in WB, IHC, ICC.

Anti-EWS Antibody SKU: 540-EWS

Bulk Order Anti-EWS Antibody

Product Details

Product Specific Protocols

General References

Anti-EWS Antibody
SKU: 540-EWS