Our Anti-EphrinB (Tyr331) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat EphrinB (Tyr331) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat testes lysate showing specific immunolabeling of the ~46 kDa EphrinB phosphorylated at Tyr331 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1 mg protein for 30 min).
EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work demonstrated that Tyr331 of EphrinB was phosphorylated in HEK293 cells after stimulation by the soluble EphB2 receptor tyrosine kinase (Kalo et al., 2001).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
EPHB2
46 kDa
Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr331 of chicken EphrinB, conjugated to keyhole limpet hemocyanin (KLH). Note: Chicken Tyr331 is the homolog of mouse and rat Tyr343, human Tyr344 and also Xenopus Tyr324.
Mouse, Rat
Bovine, Chicken, Human, Xenopus, Zebrafish
AB_2492088
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~46 kDa EphrinB protein phosphorylated at Tyr331. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Tyr331
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Bong, Y.S., Park, Y.H., Lee, H.S., Mood, K., Ishimura, A. and Daar, I.O., 2004. Tyr-298 in ephrinB1 is critical for an interaction with the Grb4 adaptor protein. Biochemical Journal, 377(2), pp.499-507. PMID: 14535844
Dufour, A., Seibt, J., Passante, L., Depaepe, V., Ciossek, T., Frisén, J., Kullander, K., Flanagan, J.G., Polleux, F. and Vanderhaeghen, P., 2003. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes. Neuron, 39(3), pp.453-465. PMID: 12895420
Flanagan, J.G. and Vanderhaeghen, P., 1998. The ephrins and Eph receptors in neural development. Annual Review of Neuroscience, 21(1), pp.309-345. PMID: 9530499
Oike, Y., Ito, Y., Hamada, K., Zhang, X.Q., Miyata, K., Arai, F., Inada, T., Araki, K., Nakagata, N., Takeya, M. and Kisanuki, Y.Y., 2002. Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells. Blood, 100(4), pp.1326-1333. PMID: 12149214
Palmer, A., Zimmer, M., Erdmann, K.S., Eulenburg, V., Porthin, A., Heumann, R., Deutsch, U. and Klein, R., 2002. EphrinB phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL phosphatase. Molecular Cell, 9(4), pp.725-737. PMID: 11983165
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