Anti-EphA4 (Tyr-602) [conserved site], Phosphospecific Antibody

Name: Anti-EphA4 (Tyr-602) [conserved site], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: EP2731
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

13 Citations

Product Type

Primary Antibody

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

EPHA4

Molecular Weight

120

Go to Full Product Details

Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).

Paraformaldehyde-fixed zebrafish embryos were probed with anti-EphA4 (Tyr-602) (EP2731) then detected using Alexa Fluor 647 goat anti-rabbit. Arrows show labeling of somite boundaries and the notochord. (Image provided by Dr. Scott Holley at the Department of Molecular, Cellular and Developmental Biology, Yale University.)

Volume: 100 µL

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Product Details

Target

EphA4 (Tyr-602))

Target Description

The Eph family of Receptor tyrosine kinases and their Ephrin ligands are important for cell positioning and morphogenesis during development. Eph receptors are classified into 10 EphA and 6 EphB receptors, which preferentially bind to the type A and type B ephrins, respectively. The EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. EphA4 signaling pathways require its kinase activity and involve binding and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). EphA4 activation leads autophosphorylation of Tyr-596 and Tyr-602, and the conserved sites in EphA2 are required for binding to the GEFs, Vav2 and Vav3, and ephrin-induced cell migration. The Tyr-779 site in the kinase domain is also phosphorylated in vivo and may regulate kinase activity. Activated EphA4 leads to Src kinase phosphorylation of the GEF, ephexin-1, and this activates RhoA. Thus, EphA4 signaling involves complex tyrosine phosphorylation in its cytoplasmic region along with interaction with several GEFs.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

EPHA4

Molecular Weight

120

Antigen

EphA4 (Tyr-602) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding phosphorylated tyrosine 602 in human EphA4. This sequence has significant homology to the conserved site in rat and mouse EphA4, and is conserved well in most other EphA and EphB family members.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to unrelated phosphotyrosine peptides before affinity purification using EphA4 (Tyr-602) peptide. The purified antibody detects a 120 kDa* band corresponding to EphA4 in Western blots of HUVEC cells treated with pervanadate and in mouse brain tissue.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-602

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SEK, Eph

UniProt Details

UniProt (Human): P54764
UniProt (Immunogen Species): P54764

Product Specific References for Applications and Species

Immunocytochemistry: Chicken | Mouse
Immunohistochemistry: Mouse | Zebrafish
Western Blot: Chicken | Mouse | Rat

Immunocytochemistry: Chicken
PMID	Dilution	Publication
30237102	1:1000	Fiore, L, et al. 2019. Regulation of axonal EphA4 forward signaling is involved in the effect of EphA3 on chicken retinal ganglion cell axon growth during retinotectal mapping. Experimental eye research, 46-60.
22685584	not listed	Ortalli, AL, et al. 2012. EphA3 expressed in the chicken tectum stimulates nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation. PLoS one, e38566.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
31011859	1:50	Garcia-Diaz, B, et al. 2019. Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling. Acta neuropathologica, 457-476.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
37485875	1:100	Yao, Z, et al. 2023. Age-related Decline in Hippocampal Tyrosine Phosphatase PTPRO is a Mechanistic Factor in Chemotherapy-related Cognitive Impairment. JCI Insight, e166306.
37485875	1:100	Yao, Z, et al. 2023. Age-related Decline in Hippocampal Tyrosine Phosphatase PTPRO is a Mechanistic Factor in Chemotherapy-related Cognitive Impairment. JCI Insight, e166306.

Immunohistochemistry: Zebrafish
PMID	Dilution	Publication
19641014	1:100	Jülich, D, et al. 2009. Control of extracellular matrix assembly along tissue boundaries via Integrin and Eph/Ephrin signaling. Development, 2913-2921.

Western Blot: Chicken
PMID	Dilution	Publication
30237102	1:1000	Fiore, L, et al. 2019. Regulation of axonal EphA4 forward signaling is involved in the effect of EphA3 on chicken retinal ganglion cell axon growth during retinotectal mapping. Experimental eye research, 46-60.

Western Blot: Mouse
PMID	Dilution	Publication
37485875	1:1000	Yao, Z, et al. 2023. Age-related Decline in Hippocampal Tyrosine Phosphatase PTPRO is a Mechanistic Factor in Chemotherapy-related Cognitive Impairment. JCI Insight, e166306.
37485875	1:1000	Yao, Z, et al. 2023. Age-related Decline in Hippocampal Tyrosine Phosphatase PTPRO is a Mechanistic Factor in Chemotherapy-related Cognitive Impairment. JCI Insight, e166306.
31011859	1:300	Garcia-Diaz, B, et al. 2019. Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling. Acta neuropathologica, 457-476.
29114064	not listed	Huang, T, et al. 2017. SORLA attenuates EphA4 signaling and amyloid β-induced neurodegeneration. The Journal of experimental medicine, 3669-3685.

Western Blot: Rat
PMID	Dilution	Publication
24958880	not listed	Fu, AKY, et al. 2014. Blockade of EphA4 signaling ameliorates hippocampal synaptic dysfunctions in mouse models of Alzheimer's disease. Proceedings of the National Academy of Sciences of the United States of America, 9959-9964.
24658113	not listed	Vargas, LM, et al. 2014. EphA4 activation of c-Abl mediates synaptic loss and LTP blockade caused by amyloid-β oligomers. PLoS one, e92309.

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Anti-EphA4 (Tyr-602) [conserved site], Phosphospecific Antibody