Name: Anti-Elk-1 (Ser383) Antibody
Brand: PhosphoSolutions
SKU: p155-383
Price: 415.0 USD
Availability: InStock

Our Anti-Elk-1 (Ser383) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Elk-1 (Ser383) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Canine, Human, Non-Human Primate, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

ELK1

Molecular Weight

46 kDa

Go to Full Product Details

Western blot of recombinant Elk-1 showing specific immunolabeling of the ~46 kDa Elk-1 phosphorylated at Ser383 in the right lane (-). Phosphospecificity is shown in the left lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Elk-1 (Ser383) Antibody

Product Details

Target

Elk-1 (Ser383)

Target Description

Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser-383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

ELK1

Molecular Weight

46 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 of human Elk-1, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Canine, Human, Non-Human Primate, Zebrafish

Antibody Registry ID

AB_2492085

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for ~46 kDa Elk-1 phosphorylated at Ser383. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser383

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

ELK 1 antibody, Elk1 antibody, ELK1 member of ETS oncogene family antibody, ELK1 protein antibody, ELK1, ETS transcription factor antibody, ELK1_HUMAN antibody, ELK2 member of ETS oncogene family antibody, ETS domain containing protein Elk 1 antibody, ETS domain containing protein Elk1 antibody, ETS domain protein Elk1 antibody, ETS domain-containing protein Elk-1 antibody, ETS like gene 1 antibody, Member of ETS oncogene family antibody, Oncogene Elk1 antibody, Tyrosine kinase (ELK1) oncogene antibody

UniProt Details

UniProt (Human): P19419
UniProt (Immunogen Species): P19419

NCBI Gene ID

2002

Product Specific Protocols

Western Blot Protocol: Download

General References

Hill, C.S. and Treisman, R., 1995. Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell, 80(2), pp.199-211. PMID: 8521730
Li, Q.J., Yang, S.H., Maeda, Y., Sladek, F.M., Sharrocks, A.D. and Martins‐Green, M., 2003. MAP kinase phosphorylation‐dependent activation of Elk‐1 leads to activation of the co‐activator p300. The EMBO Journal, 22(2), pp.281-291. PMID: 8521730
Thiels, E., Kanterewicz, B.I., Norman, E.D., Trzaskos, J.M. and Klann, E., 2002. Long-term depression in the adult hippocampus in vivo involves activation of extracellular signal-regulated kinase and phosphorylation of Elk-1. Journal of Neuroscience, 22(6), pp.2054-2062. PMID: 8521730

Recover password

Create my account

Anti-Elk-1 (Ser383) Antibody

Our Anti-Elk-1 (Ser383) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Elk-1 (Ser383) and is antigen affinity purified from pooled serum. It is great for use in WB.