Our Anti-Elk-1 (Ser383) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Elk-1 (Ser383) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of recombinant Elk-1 showing specific immunolabeling of the ~46 kDa Elk-1 phosphorylated at Ser383 in the right lane (-). Phosphospecificity is shown in the left lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Anti-Elk-1 (Ser383) Antibody
Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser-383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).
Antigen Affinity Purified from Pooled Serum
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 of human Elk-1, conjugated to keyhole limpet hemocyanin (KLH).
Canine, Human, Non-Human Primate, Zebrafish
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Specific for ~46 kDa Elk-1 phosphorylated at Ser383. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
ELK 1 antibody, Elk1 antibody, ELK1 member of ETS oncogene family antibody, ELK1 protein antibody, ELK1, ETS transcription factor antibody, ELK1_HUMAN antibody, ELK2 member of ETS oncogene family antibody, ETS domain containing protein Elk 1 antibody, ETS domain containing protein Elk1 antibody, ETS domain protein Elk1 antibody, ETS domain-containing protein Elk-1 antibody, ETS like gene 1 antibody, Member of ETS oncogene family antibody, Oncogene Elk1 antibody, Tyrosine kinase (ELK1) oncogene antibody
Hill, C.S. and Treisman, R., 1995. Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell, 80(2), pp.199-211. PMID: 8521730
Li, Q.J., Yang, S.H., Maeda, Y., Sladek, F.M., Sharrocks, A.D. and Martins‐Green, M., 2003. MAP kinase phosphorylation‐dependent activation of Elk‐1 leads to activation of the co‐activator p300. The EMBO Journal, 22(2), pp.281-291. PMID: 8521730
Thiels, E., Kanterewicz, B.I., Norman, E.D., Trzaskos, J.M. and Klann, E., 2002. Long-term depression in the adult hippocampus in vivo involves activation of extracellular signal-regulated kinase and phosphorylation of Elk-1. Journal of Neuroscience, 22(6), pp.2054-2062. PMID: 8521730
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