Anti-EB3 (Ser-162), Phosphospecific Antibody

Name: Anti-EB3 (Ser-162), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: EP4981
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

MAPRE3

Molecular Weight

Go to Full Product Details

Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (EM5091) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).

Volume: 100 µL

Variant

Price:

$309

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Bulk Order Anti-EB3 (Ser-162), Phosphospecific Antibody

Product Details

Target

EB3 (Ser-162)

Target Description

Microtubles (MTs) are oriented with a fast growing plus-end and a slower growing minus-end. The MT plus-end is a crucial site for the regulation of MT dynamics and MT association with different cellular organelles by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The End-Binding (EB) family of +TIPs includes EB1 (MAPRE1), EB2 (MAPRE2, RP1), and EB3 (MAPRE3, EBF3). EB proteins are ubiquitiously expressed +TIPs that can dimerize through a coiled-coil C-terminal region, and bind MTs through an N-terminal calponin homology domain. EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. Site specific phosphorylation may regulate EB activity. EB3 Ser-162 phosphorylation destabilizes EB3 dimer and reduces MT growth, while aurora-kinase induced Ser-176 phosphorylation regulates EB3 protein stability during mitosis.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

MAPRE3

Molecular Weight

Antigen

Phospho-EB3 (Ser-162) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 162 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3, and is not conserved in EB1 and EB2.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 32 kDa* protein corresponding to the apparent molecular mass of phosphorylated EB3 on SDS-PAGE immunoblots of mouse brain. This reactivity is significantly reduced after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-162

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Microtubule RP/EB, MAPRE3, APC, EB3, End-binding. RP3

UniProt Details

UniProt (Human): Q9UPY8
UniProt (Immunogen Species): Q9UPY8

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Anti-EB3 (Ser-162), Phosphospecific Antibody