Anti-EB1 (C-terminal region) Antibody (KT51)

Name: Anti-EB1 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: EM5041
Price: 340.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

KT51

Applications

ICC, WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

MAPRE1

Molecular Weight

Go to Full Product Details

Immunocytochemical labeling of EB1 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-α-Tubulin (TM4111) (left) and rat monoclonal anti-EB1 (EM5041) (middle). The antibodies were detected using either goat anti-mouse:DyLight® 488 (MS3011) or goat anti-Rat:DyLight® 594 (RS3111).

Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals EM5041 anti-EB1 (lane 1), EM5061 anti-EB1/2/3 (lane 2), EM5081 anti-EB2 (lane 3), EM5101 anti-EB3 (lane 4), and EM5091 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific:HRP (RS3121).

Volume: 100 µL

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Product Details

Target

EB1 (C-terminal region)

Target Description

The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (α) and short (β) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP). The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility.

Format

Protein G Purified

Clonality

Monoclonal

Clone

KT51

Isotype

IgG2a

Applications

ICC, WB

Host Species

Mouse

Gene Name

MAPRE1

Molecular Weight

Antigen

Clone KT51 was generated from a GST fusion protein containing amino acids from the C-terminal region of mouse EB1. This sequence is highly conserved in human and rat EB1.

Species Reactivity

Human, Mouse, Rat

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.05% NaN3

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects a 30 kDa* protein corresponding to the molecular mass of EB1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB1 by immunocytochemistry in HeLa and A431 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Microtubule RP/EB, MAPRE1, APC, EB1, End-binding

UniProt Details

UniProt (Human): Q15691
UniProt (Immunogen Species): Q61166

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Anti-EB1 (C-terminal region) Antibody (KT51)

Anti-EB1 (C-terminal region) Antibody
SKU: EM5041

Bulk Order Anti-EB1 (C-terminal region) Antibody

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Anti-EB1 (C-terminal region) Antibody (KT51)

Anti-EB1 (C-terminal region) Antibody SKU: EM5041

Bulk Order Anti-EB1 (C-terminal region) Antibody

Product Details

Anti-EB1 (C-terminal region) Antibody
SKU: EM5041