Anti-Early Endosome Antigen 1 (EEA1) Antibody (M347)

Name: Anti-Early Endosome Antigen 1 (EEA1) Antibody
Brand: PhosphoSolutions
SKU: EM3471
Price: 360.0 USD
Availability: InStock

Our Early Endosome Antigen 1 (EEA1) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Early Endosome Antigen 1 (EEA1) and is Protein A purified. It is great for use in WB, ICC.

Immunocytochemical labeling of EEA1 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal EEA1 (EM3471). The antibody was detected using goat anti-mouse DyLight® 488.

Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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SKU: EM3471

Product Details

Target

Early Endosome Antigen 1 (EEA1)

Target Description

Early endosomes are cellular compartments that receive endocytosed materials and sort them for vesicular transport to late endosomes and lysosomes, as well as for recycling material to the plasma membrane. Early endosome antigen 1 (EEA1) is an early endosomal protein that contains an N-terminal zinc finger motif, a cys-rich C-terminal metal-binding finger, and multiple sites for N-glycosylation, phosphorylation, and N-myristoylation. Expression of EEA1 mRNA is observed in skeletal muscle, heart, brain, lung, liver, and pancreas. Immunoblot analysis shows that EEA1 is a 180-kDa protein localized in membrane and cytosolic fractions. Immunofluorescence microscopy shows that EEA1 colocalizes with transferrin and with RAB5 in early endosomes, but not with RAB7 in late endosomes.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M347

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

EEA1

Molecular Weight

180

Antigen

Clone M347 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human EEA1. This sequence has high homology to similar regions in rat and mouse EEA1.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50

Conjugate

Unconjugated

Specificity

This antibody detects a 180 kDa* protein corresponding to the apparent molecular mass of EEA1 on SDS-PAGE immunoblots of adult mouse brain. In immunocytochemistry, anti-EEA1 specifically stains endosomal vesicles.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

UniProt Details

UniProt (Human): Q15075
UniProt (Immunogen Species): Q15075

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Anti-Early Endosome Antigen 1 (EEA1) Antibody (M347)

SKU: EM3471

Product Details

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