Our Early Endosome Antigen 1 (EEA1) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Early Endosome Antigen 1 (EEA1) and is Protein A purified. It is great for use in WB, ICC.
Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).
Early endosomes are cellular compartments that receive endocytosed materials and sort them for vesicular transport to late endosomes and lysosomes, as well as for recycling material to the plasma membrane. Early endosome antigen 1 (EEA1) is an early endosomal protein that contains an N-terminal zinc finger motif, a cys-rich C-terminal metal-binding finger, and multiple sites for N-glycosylation, phosphorylation, and N-myristoylation. Expression of EEA1 mRNA is observed in skeletal muscle, heart, brain, lung, liver, and pancreas. Immunoblot analysis shows that EEA1 is a 180-kDa protein localized in membrane and cytosolic fractions. Immunofluorescence microscopy shows that EEA1 colocalizes with transferrin and with RAB5 in early endosomes, but not with RAB7 in late endosomes.
Protein A Purified
Clone M347 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human EEA1. This sequence has high homology to similar regions in rat and mouse EEA1.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
This antibody detects a 180 kDa* protein corresponding to the apparent molecular mass of EEA1 on SDS-PAGE immunoblots of adult mouse brain. In immunocytochemistry, anti-EEA1 specifically stains endosomal vesicles.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.