Anti-E-Cadherin (C-terminal fragment) Antibody (M533)

Name: Anti-E-Cadherin (C-terminal fragment) Antibody
Brand: PhosphoSolutions
SKU: CM5331
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M533

Applications

Host Species

Mouse

Format

Protein G Purified

Gene Name

CDH1

Molecular Weight

120

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Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-E-Cadherin (Cytoplasmic) at 1:1000 (lane 1) and 1:4000 (lane 2) and anti-E-Cadherin (C-terminal fragment) at 1:250 (lane 3) and 1:1000 (lane 4).

Volume: 100 µL

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$255

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Product Details

Target

E-Cadherin (C-terminal fragment)

Target Description

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M533

Isotype

IgG1

Applications

Host Species

Mouse

Gene Name

CDH1

Molecular Weight

120

Antigen

Clone (M533) was generated from a mouse recombinant E-Cadherin protein containing amino acids in the C-terminal region. This sequence is highly conserved in human and rat E-cadherin.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:250

Conjugate

Unconjugated

Specificity

The E-cadherin antibody detects a 120 kDa* protein corresponding to endogenous E-Cadherin expressed in human A431 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Uvomorulin, Cadherin-1, CTF1, CTF2, CTF3, CD324, Epithelial Cadherin

UniProt Details

UniProt (Human): P12830
UniProt (Immunogen Species): P09803

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Anti-E-Cadherin (C-terminal fragment) Antibody (M533)

Anti-E-Cadherin (C-terminal fragment) Antibody
SKU: CM5331

Bulk Order Anti-E-Cadherin (C-terminal fragment) Antibody

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Anti-E-Cadherin (C-terminal fragment) Antibody (M533)

Anti-E-Cadherin (C-terminal fragment) Antibody SKU: CM5331

Bulk Order Anti-E-Cadherin (C-terminal fragment) Antibody

Product Details

Anti-E-Cadherin (C-terminal fragment) Antibody
SKU: CM5331