Name: Anti-Dynamin (Ser778) Antibody
Brand: PhosphoSolutions
SKU: p1050-778
Price: 415.0 USD
Availability: InStock

Our Anti-Dynamin (Ser778) sheep polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Dynamin (Ser778) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human

Applications

WB

Host Species

Sheep

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

DNM1

Molecular Weight

95 kDa

Go to Full Product Details

Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at Ser778 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Dynamin (Ser778) Antibody

Product Details

Target

Dynamin (Ser778)

Target Description

Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser-774 and Ser-778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Sheep

Gene Name

DNM1

Molecular Weight

95 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 of rat dynamin, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human

Antibody Registry ID

AB_2492083

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled sheep serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~95 kDa dynamin protein phosphorylated at Ser778. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser778

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

B dynamin antibody, D100 antibody, DNM 1 antibody, DNM antibody, DNM1 antibody, DYN1_HUMAN antibody, Dynamin antibody, Dynamin-1 antibody, Dynamin1 antibody

UniProt Details

UniProt (Human): Q05193
UniProt (Immunogen Species): P21575

NCBI Gene ID

140694

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
28052246	not listed	Vargas, K.J., et al. 2017. Synucleins have multiple effects on presynaptic architecture. Cell Reports, 18(1), pp.161-173.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Dynamin (Ser778) Antibody

Our Anti-Dynamin (Ser778) sheep polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Dynamin (Ser778) and is antigen affinity purified from pooled serum. It is great for use in WB.