Name: Anti-Dynamin Antibody
Brand: PhosphoSolutions
SKU: 480-DYN
Price: 415.0 USD
Availability: InStock

Our Anti-Dynamin rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Dynamin and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Bovine, Chicken, Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

DNM1

Molecular Weight

95 kDa

Go to Full Product Details

Western Blot of rat cortical lysate showing labeling of the ~95 kDa dynamin protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Dynamin Antibody

Product Details

Target

Dynamin

Target Description

Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). There are 3 known isoforms of Dynamin, each having several splice variants as well. Dynamin I is expressed only in neurons whereas Dynamin II is ubiquitously expressed and Dynamin III is found primarily in the testes. Dynamin 1 is phosphorylated by PKC and dephosphorylated by calcineurin.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

DNM1

Molecular Weight

95 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the N-terminal region of human dynamin, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Bovine, Chicken, Non-Human Primate

Antibody Registry ID

AB_2492081

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the N-terminal peptide used as antigen.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~95 kDa dynamin protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

B dynamin antibody, D100 antibody, DNM 1 antibody, DNM antibody, DNM1 antibody, DYN1_HUMAN antibody, Dynamin antibody, Dynamin-1 antibody, Dynamin1 antibody

UniProt Details

UniProt (Human): Q05193
UniProt (Immunogen Species): Q05193

NCBI Gene ID

1759

Product Specific Protocols

Western Blot Protocol: Download

General References

Cousin, M.A., Tan, T.C. and Robinson, P.J., 2001. Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin. Journal of Neurochemistry, 76(1), pp.105-116. PMID: 11145983
Graham, M.E., O'Callaghan, D.W., McMahon, H.T. and Burgoyne, R.D., 2002. Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size. Proceedings of the National Academy of Sciences, 99(10), pp.7124-7129. PMID: 11997474
Tan, T.C., Valova, V.A., Malladi, C.S., Graham, M.E., Berven, L.A., Jupp, O.J., Hansra, G., McClure, S.J., Sarcevic, B., Boadle, R.A. and Larsen, M.R., 2003. Cdk5 is essential for synaptic vesicle endocytosis. Nature Cell Biology, 5(8), p.701. PMID: 12855954
Tsuboi, T., Terakawa, S., Scalettar, B.A., Fantus, C., Roder, J. and Jeromin, A., 2002. Sweeping model of dynamin activity - Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins. Journal of Biological Chemistry, 277(18), pp.15957-15961. PMID: 11856729

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Anti-Dynamin Antibody

Our Anti-Dynamin rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Dynamin and is antigen affinity purified from pooled serum. It is great for use in WB.