Name: Anti-Dopamine Transporter (Thr53) Antibody
Brand: PhosphoSolutions
SKU: p435-53
Price: 415.0 USD
Availability: InStock

Our Anti-Dopamine Transporter (Thr53) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Dopamine Transporter (Thr53) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SLC6A3

Molecular Weight

55 kDa

Go to Full Product Details

Western blot of rat striatal lysate showing specific immunolabeling of the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Dopamine Transporter (Thr53) Antibody

Product Details

Target

Dopamine Transporter (Thr53)

Target Description

The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996). It has been shown that phosphorylation at Thr-53 directly affects dopamine influx and amphetamine-stimulated substrate efflux, indicating that the Thr-53 residue plays a major role in transport activity (Foster et al., 2012).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

SLC6A3

Molecular Weight

55 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of rat dopamine transporter, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2492078

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the DAT protein. The antibody works best in lysates that have not been boiled prior to being run on an SDS-PAGE gel. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr53

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DA transporter antibody, DAT 1 antibody, DAT antibody, DAT1 antibody, Dopamine transporter 1 antibody, Dopamine transporter antibody, PKDYS antibody, SC6A3_HUMAN antibody, SLC6A3 antibody, Sodium dependent dopamine transporter antibody, Sodium-dependent dopamine transporter antibody, Solute carrier family 6 (neurotransmitter transporter dopamine) member 3 antibody, Solute carrier family 6 (neurotransmitter transporter) member 3 antibody, Solute carrier family 6 member 3 antibody, Variable number tandem repeat (VNTR) antibody

UniProt Details

UniProt (Immunogen Species): P23977

NCBI Gene ID

24898

Product Specific References for Applications and Species

Western Blot: Mouse | Rat

Western Blot: Mouse
PMID	Dilution	Publication
33794225	1:2000	Varman, D.R., et al. 2021. Novelty-induced Hyperactivity and Suppressed Cocaine Induced Locomotor Activation in Mice Lacking Threonine 53 Phosphorylation of Dopamine Transporter. Behavioral Brain Research,/i., p.113267.
33599293	not listed	Asami, M., et al. 2021. Dopamine and the phosphorylated dopamine transporter are increased in the diacylglycerol kinase ƞ‐knockout mouse brain. FEBS letters.
33032296	not listed	Alonso, I.P., et al. 2020. Dopamine transporter function fluctuates across sleep/wake state: potential impact for addiction. Neuropsychopharmacology, 46(4), pp.699-708.
29739866	not listed	Gowrishankar, R., et al. 2018. Region-specific regulation of presynaptic dopamine homeostasis by D2 autoreceptors shapes the in vivo impact of the neuropsychiatric disease-associated DAT variant Val559. Journal of Neuroscience, 38(23), pp.5302-5312.
29486157	not listed	Lu, Q., et al. 2018. Abnormalities of the serotonergic system in diacylglycerol kinase δ-deficient mouse brain. Biochemical and Biophysical Research Communications, 497(4), pp.1031-1037.

Western Blot: Rat
PMID	Dilution	Publication
36301217	1:1000	Samson, K.R., et al. 2022. Intermittent Access to Oxycodone Decreases Dopamine Uptake in the Nucleus Accumbens Core During Abstinence. Addiction Biology, e13241.
30071195	1:1000	Brodnik, Z.D., et al. 2018. Hypocretin receptor 1 involvement in cocaine-associated behavior: therapeutic potential and novel mechanistic insights. Brain Research, p.145894.
26941626	1:5000	Aher, Y.D., et al. 2016. A novel heterocyclic compound CE-104 enhances spatial working memory in the radial arm maze in rats and modulates the dopaminergic system. Frontiers in Behavioral Neuroscience, 10, p.20.
26407764	1:5000	Sase, A., et al. 2016. A heterocyclic compound CE-103 inhibits dopamine reuptake and modulates dopamine transporter and dopamine D1-D3 containing receptor complexes. Neuropharmacology, 102, pp.186-196.
22722938	not listed	Foster, J.D., et al. 2012. Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux. Journal of Biological Chemistry, 287(35), pp.29702-29712.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Dopamine Transporter (Thr53) Antibody

Our Anti-Dopamine Transporter (Thr53) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Dopamine Transporter (Thr53) and is antigen affinity purified from pooled serum. It is great for use in WB.