Our Anti-DOPA Decarboxylase rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects bovine, canine, guinea pig, human, rabbit, rat, and sheep DOPA Decarboxylase and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of 5 µg of bovine adrenal medulla lysate showing specific immunolabeling of the ~55 kDa DOPA decarboxylase protein.
Anti-DOPA Decarboxylase Antibody
DOPA decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).
Antigen Affinity Purified from Pooled Serum
Synthetic peptide from N-terminus region of human DOPA decarboxylase (DDC), conjugated to keyhole limpet hemocyanin (KLH).
Bovine, Canine, Guinea Pig, Human, Rabbit, Rat, Sheep
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from pooled rabbit serum by affinity purification using an AminoLink® Plus column matrix to which purified, recombinant bovine DDC was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB Brain: 1:1000
Specific for endogenous levels of the ~55 kDa DDC protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.