Name: Anti-DGCR8 (Ser377) Antibody
Brand: PhosphoSolutions
SKU: p1035-377
Price: 415.0 USD
Availability: InStock

Our Anti-DGCR8 (Ser377) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse DGCR8 (Ser377) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

DGCR8

Molecular Weight

120 kDa

Go to Full Product Details

Western blot of mouse nuclei lysate showing specific immunolabeling of the ~120 kDa DGCR8 protein phosphorylated at in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-DGCR8 (Ser377) Antibody

Product Details

Target

DGCR8 (Ser377)

Target Description

The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han, J., et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller, M., et al, 2010).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

DGCR8

Molecular Weight

120 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 of human DGCR8, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_2492071

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phospho peptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~120 kDa DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser377

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DGCRK6 antibody, C22orf12 antibody, D16H22S788E antibody, D16Wis2 antibody, DGCR 8 antibody, Dgcr8 antibody, DGCR8 microprocessor complex subunit antibody, DGCR8_HUMAN antibody, DGCRK 6 antibody, DiGeorge syndrome critical region 8 antibody, DiGeorge syndrome critical region gene 8 antibody, Gy1 antibody, Microprocessor complex subunit DGCR8 antibody, pasha antibody

UniProt Details

UniProt (Human): Q8WYQ5
UniProt (Immunogen Species): Q8WYQ5

NCBI Gene ID

1644

Product Specific Protocols

Western Blot Protocol: Download

General References

Han, J., Lee, Y., Yeom, K.H., Nam, J.W., Heo, I., Rhee, J.K., Sohn, S.Y., Cho, Y., Zhang, B.T. and Kim, V.N., 2006. Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. Cell, 125(5), pp.887-901. PMID: 16751099
Faller, M., Toso, D., Matsunaga, M., Atanasov, I., Senturia, R., Chen, Y., Zhou, Z.H. and Guo, F., 2010. DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures. RNA, 16(8), pp.1570-1583. PMID: 20558544

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Anti-DGCR8 (Ser377) Antibody

Our Anti-DGCR8 (Ser377) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse DGCR8 (Ser377) and is antigen affinity purified from pooled serum. It is great for use in WB.