Our Anti-DARPP-32 (Thr75) phosphospecific primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat DARPP-32 (Thr75) and is antigen affinity purified. It is great for use in WB.
Primary Antibody
Mouse, Rat
Bovine, Canine, Chicken, Human, Non-Human Primate
WB
Rabbit
Antigen Affinity Purified
PPP1R1B
32 kDa

Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr75 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Product Specific References for Applications and Species
- Western Blot: Mouse
Western Blot: Mouse | ||
PMID | Dilution | Publication |
21946266 | not listed | Errico, F., et al. 2011. Higher free D-aspartate and N-methyl-D-aspartate levels prevent striatal depotentiation and anticipate L-DOPA-induced dyskinesia. Experimental neurology, 232(2), pp.240-250. |
20720111 | 1:1000 | Napolitano, F., et al. 2010. Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine. Journal of Neuroscience, 30(33), pp.11043-11056. |
18234897 | 1:1000 | Niculescu, M., et al. 2008. Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice. Journal of Neuroscience, 28(5), pp.1198-1207. |
Product Specific Protocols
- Western Blot Protocol: Download