Anti-DARPP-32 (Thr34) Antibody

Name: Anti-DARPP-32 (Thr34) Antibody
Brand: PhosphoSolutions
SKU: p1025-34
Price: 399.0 USD
Availability: InStock

from PhosphoSolutions Datasheet Trial Size Pooled Serum

11 Citations

Our Anti-DARPP-32 (Thr34) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat DARPP-32 (Thr34) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

PPP1R1B

Molecular Weight

32 kDa

Go to Full Product Details

Effects of striatal slice treatment with NMDA (25 μM, 5 min), dopamine (DA, 10 μM, 15 min), or both on PKA-dependent phosphorylation of Ser845 GluR1 (top) and Thr34 DARPP-32 (bottom). Image from publication CC-BY-4.0.

Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr34 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$399

Quantity:

Bulk Order Anti-DARPP-32 (Thr34) Antibody

Product Details

Target

DARPP-32 (Thr34)

Target Description

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

PPP1R1B

Molecular Weight

32 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate

Antibody Registry ID

AB_2492068

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr34. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr34

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DARPP32 antibody, DARPP-32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody, Dopamine and cAMP regulated phosphoprotein DARPP32 antibody, Dopamine- and cAMP-regulated neuronal phosphoprotein antibody, FLJ20940 antibody, IPPD antibody, Neuronal phosphoprotein DARPP 32 antibody, PPP1R1B antibody, PPR1B_HUMAN antibody, Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody, Protein phosphatase 1 regulatory inhibitor subunit 1B antibody, Protein phosphatase 1 regulatory subunit 1B antibody

UniProt Details

UniProt (Human): Q9UD71
UniProt (Immunogen Species): Q6J4I0

NCBI Gene ID

360616

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
26947946	not listed	Bonito-Olivia, A., et al. 2016. Differential regulation of the phosphorylation of Trimethyl-lysine27 histone H3 at serine 28 in distinct populations of striatal projection neurons. Neuropharmacology, 107, pp.89-99.
26657176	not listed	Bonito-Olivia, A., et al. 2016. Involvement of the striatal medium spiny neurons of the direct pathway in the motor stimulant effects of phencyclidine. International Journal of Neuropsychopharmacology, 19(6).
26459714	not listed	Del’Guidice, T., et al. 2015. Selective disruption of dopamine D2-receptors/beta-arrestin2 signaling by mood stabilizers. Journal of Receptors and Signal Transduction, 1-9. Jun; 35(3):224-32.
26320830	not listed	Beaulieu, J.M., et al. 2016. In vivo veritas, the next frontier for functionally selective GPCR ligands. Methods, 92, pp.64-71.
26240334	not listed	Del’Guidice, T., et al. 2015. FXR1P is a GSK3β substrate regulating mood and emotion processing. Proceedings of the National Academy of Sciences, 112(33), pp.E4610-E4619.
21946266	not listed	Errico, F., et al. 2011. Higher free D-aspartate and N-methyl-D-aspartate levels prevent striatal depotentiation and anticipate L-DOPA-induced dyskinesia. Experimental neurology, 232(2), pp.240-250.
21372109	not listed	Marazziti, D., et al. 2011. Absence of the GPR37/PAEL receptor impairs striatal Akt and ERK2 phosphorylation, ΔFosB expression, and conditioned place preference to amphetamine and cocaine. The FASEB Journal, 25(6), pp.2071-2081.
20720111	1:1000	Napolitano, F., et al. 2010. Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine. Journal of Neuroscience, 30(33), pp.11043-11056.
19181855	not listed	Maiya, R., et al. 2009. Tissue plasminogen activator modulates the cellular and behavioral response to cocaine. Proceedings of the National Academy of Sciences, 106(6), pp.1983-1988.
18234897	1:1000	Niculescu, M., et al. 2008. Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice. Journal of Neuroscience, 28(5), pp.1198-1207.
16954211	not listed	Beaulieu, J.M., et al. 2006. Paradoxical striatal cellular signaling responses to psychostimulants in hyperactive mice. Journal of Biological Chemistry, 281(43), pp.32072-32080.

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Anti-DARPP-32 (Thr34) Antibody

Our Anti-DARPP-32 (Thr34) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat DARPP-32 (Thr34) and is antigen affinity purified from pooled serum. It is great for use in WB.