Our Anti-DARPP-32 (Thr34) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat DARPP-32 (Thr34) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr34 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Anti-DARPP-32 (Thr34) Antibody
SKU: p1025-34
Product Details
DARPP-32 (Thr34)
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
PPP1R1B
32 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Canine, Chicken, Human, Non-Human Primate
AB_2492068
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr34. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Thr34
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
DARPP32 antibody, DARPP-32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody, Dopamine and cAMP regulated phosphoprotein DARPP32 antibody, Dopamine- and cAMP-regulated neuronal phosphoprotein antibody, FLJ20940 antibody, IPPD antibody, Neuronal phosphoprotein DARPP 32 antibody, PPP1R1B antibody, PPR1B_HUMAN antibody, Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody, Protein phosphatase 1 regulatory inhibitor subunit 1B antibody, Protein phosphatase 1 regulatory subunit 1B antibody
Bonito-Olivia, A., et al. 2016. Differential regulation of the phosphorylation of Trimethyl-lysine27 histone H3 at serine 28 in distinct populations of striatal projection neurons. Neuropharmacology, 107, pp.89-99.
Bonito-Olivia, A., et al. 2016. Involvement of the striatal medium spiny neurons of the direct pathway in the motor stimulant effects of phencyclidine. International Journal of Neuropsychopharmacology, 19(6).
Del’Guidice, T., et al. 2015. Selective disruption of dopamine D2-receptors/beta-arrestin2 signaling by mood stabilizers. Journal of Receptors and Signal Transduction, 1-9. Jun; 35(3):224-32.
Del’Guidice, T., et al. 2015. FXR1P is a GSK3β substrate regulating mood and emotion processing. Proceedings of the National Academy of Sciences, 112(33), pp.E4610-E4619.
Marazziti, D., et al. 2011. Absence of the GPR37/PAEL receptor impairs striatal Akt and ERK2 phosphorylation, ΔFosB expression, and conditioned place preference to amphetamine and cocaine. The FASEB Journal, 25(6), pp.2071-2081.
Napolitano, F., et al. 2010. Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine. Journal of Neuroscience, 30(33), pp.11043-11056.
Maiya, R., et al. 2009. Tissue plasminogen activator modulates the cellular and behavioral response to cocaine. Proceedings of the National Academy of Sciences, 106(6), pp.1983-1988.
Niculescu, M., et al. 2008. Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice. Journal of Neuroscience, 28(5), pp.1198-1207.
