Name: Anti-DARPP-32 (Thr34) Antibody
Brand: PhosphoSolutions
SKU: p1025-34
Price: 415.0 USD
Availability: InStock

Our Anti-DARPP-32 (Thr34) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat DARPP-32 (Thr34) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

PPP1R1B

Molecular Weight

32 kDa

Go to Full Product Details

Effects of striatal slice treatment with NMDA (25 μM, 5 min), dopamine (DA, 10 μM, 15 min), or both on PKA-dependent phosphorylation of Ser845 GluR1 (top) and Thr34 DARPP-32 (bottom). Image from publication CC-BY-4.0. PMID: 35835216

Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr34 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-DARPP-32 (Thr34) Antibody

Product Details

Target

DARPP-32 (Thr34)

Target Description

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

PPP1R1B

Molecular Weight

32 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate

Antibody Registry ID

AB_2492068

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr34. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr34

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DARPP32 antibody, DARPP-32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody, Dopamine and cAMP regulated phosphoprotein DARPP32 antibody, Dopamine- and cAMP-regulated neuronal phosphoprotein antibody, FLJ20940 antibody, IPPD antibody, Neuronal phosphoprotein DARPP 32 antibody, PPP1R1B antibody, PPR1B_HUMAN antibody, Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody, Protein phosphatase 1 regulatory inhibitor subunit 1B antibody, Protein phosphatase 1 regulatory subunit 1B antibody

UniProt Details

UniProt (Human): Q9UD71
UniProt (Immunogen Species): Q6J4I0

NCBI Gene ID

360616

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat

Western Blot: Human
PMID	Dilution	Publication
35840557	1:1000	Zhang, C., et al. 2022. Autophagic sequestration of SQSTM1 disrupts the aggresome formation of ubiquitinated proteins during proteasome inhibition. Cell death & disease, 615.
29930081	not listed	Zhang, C., et al. 2018. p38δ MAPK regulates aggresome biogenesis by phosphorylating SQSTM1 in response to proteasomal stress. J Cell Sci, Jul 26;131(14).

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Western Blot: Mouse
PMID	Dilution	Publication
36918741	not listed	Choi, Y., et al. 2023. 5-Hydroxytryptophan Reduces Levodopa-Induced Dyskinesia via Regulating AKT/mTOR/S6K and CREB/ΔFosB Signals in a Mouse Model of Parkinson's Disease. Biomolecules & Therapeutics, .
26279575	not listed	Linares, J.F., et al. 2015. Amino acid activation of mTORC1 by a PB1-domain-driven kinase complex cascade. Cell reports, 12(8), pp.1339-1352.

Western Blot: Rat
PMID	Dilution	Publication
35835216	not listed	Thomas, R., et al. 2022. Integrated regulation of PKA by fast and slow neurotransmission in the nucleus accumbens controls plasticity and stress responses. The Journal of biological chemistry, 102245.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-DARPP-32 (Thr34) Antibody

Our Anti-DARPP-32 (Thr34) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat DARPP-32 (Thr34) and is antigen affinity purified from pooled serum. It is great for use in WB.