Our Anti-DARPP-32 (Thr34) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat DARPP-32 (Thr34) and is antigen affinity purified from pooled serum. It is great for use in WB.
Primary Antibody
Mouse, Rat
Bovine, Canine, Chicken, Human, Non-Human Primate
WB
Rabbit
Antigen Affinity Purified from Pooled Serum
PPP1R1B
32 kDa

Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr34 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Product Specific References for Applications and Species
- Western Blot: Mouse
Western Blot: Mouse | ||
PMID | Dilution | Publication |
26947946 | not listed | Bonito-Olivia, A., et al. 2016. Differential regulation of the phosphorylation of Trimethyl-lysine27 histone H3 at serine 28 in distinct populations of striatal projection neurons. Neuropharmacology, 107, pp.89-99. |
26657176 | not listed | Bonito-Olivia, A., et al. 2016. Involvement of the striatal medium spiny neurons of the direct pathway in the motor stimulant effects of phencyclidine. International Journal of Neuropsychopharmacology, 19(6). |
26459714 | not listed | Del’Guidice, T., et al. 2015. Selective disruption of dopamine D2-receptors/beta-arrestin2 signaling by mood stabilizers. Journal of Receptors and Signal Transduction, 1-9. Jun; 35(3):224-32. |
26320830 | not listed | Beaulieu, J.M., et al. 2016. In vivo veritas, the next frontier for functionally selective GPCR ligands. Methods, 92, pp.64-71. |
26240334 | not listed | Del’Guidice, T., et al. 2015. FXR1P is a GSK3β substrate regulating mood and emotion processing. Proceedings of the National Academy of Sciences, 112(33), pp.E4610-E4619. |
21946266 | not listed | Errico, F., et al. 2011. Higher free D-aspartate and N-methyl-D-aspartate levels prevent striatal depotentiation and anticipate L-DOPA-induced dyskinesia. Experimental neurology, 232(2), pp.240-250. |
21372109 | not listed | Marazziti, D., et al. 2011. Absence of the GPR37/PAEL receptor impairs striatal Akt and ERK2 phosphorylation, ΔFosB expression, and conditioned place preference to amphetamine and cocaine. The FASEB Journal, 25(6), pp.2071-2081. |
20720111 | 1:1000 | Napolitano, F., et al. 2010. Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine. Journal of Neuroscience, 30(33), pp.11043-11056. |
19181855 | not listed | Maiya, R., et al. 2009. Tissue plasminogen activator modulates the cellular and behavioral response to cocaine. Proceedings of the National Academy of Sciences, 106(6), pp.1983-1988. |
18234897 | 1:1000 | Niculescu, M., et al. 2008. Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice. Journal of Neuroscience, 28(5), pp.1198-1207. |
16954211 | not listed | Beaulieu, J.M., et al. 2006. Paradoxical striatal cellular signaling responses to psychostimulants in hyperactive mice. Journal of Biological Chemistry, 281(43), pp.32072-32080. |