Our Anti-DARPP-32 (Thr34) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat DARPP-32 (Thr34) and is antigen affinity purified from pooled serum. It is great for use in WB.
Primary Antibody
Mouse, Rat
Bovine, Canine, Chicken, Human, Non-Human Primate
WB
Rabbit
Antigen Affinity Purified from Pooled Serum
PPP1R1B
32 kDa


Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr34 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Product Specific References for Applications and Species
Western Blot: Human | ||
PMID | Dilution | Publication |
35840557 | 1:1000 | Zhang, C., et al. 2022. Autophagic sequestration of SQSTM1 disrupts the aggresome formation of ubiquitinated proteins during proteasome inhibition. Cell death & disease, 615. |
29930081 | not listed | Zhang, C., et al. 2018. p38δ MAPK regulates aggresome biogenesis by phosphorylating SQSTM1 in response to proteasomal stress. J Cell Sci, Jul 26;131(14). |
Western Blot: Mouse | ||
PMID | Dilution | Publication |
36918741 | not listed | Choi, Y., et al. 2023. 5-Hydroxytryptophan Reduces Levodopa-Induced Dyskinesia via Regulating AKT/mTOR/S6K and CREB/ΔFosB Signals in a Mouse Model of Parkinson's Disease. Biomolecules & Therapeutics, . | 26279575 | not listed | Linares, J.F., et al. 2015. Amino acid activation of mTORC1 by a PB1-domain-driven kinase complex cascade. Cell reports, 12(8), pp.1339-1352. |
Western Blot: Rat | ||
PMID | Dilution | Publication |
35835216 | not listed | Thomas, R., et al. 2022. Integrated regulation of PKA by fast and slow neurotransmission in the nucleus accumbens controls plasticity and stress responses. The Journal of biological chemistry, 102245. |
Product Specific Protocols
- Western Blot Protocol: Download
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