Our Anti-DARPP-32 (Ser137) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat DARPP-32 (Ser137) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Ser137 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002). Serine-137 is phosphorylated by casein kinase 1 and phosphorylation of this site is increased following acute administration of Prozac® (Svenningsson et al., 2002).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
PPP1R1B
32 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser137 of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Canine, Human, Non-Human Primate
AB_2492070
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phospho peptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Ser137. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Ser137
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Bellini, S., et al. 2018. Neuronal glutamate transporters control dopaminergic signaling and compulsive behaviors. Journal of Neuroscience, 38(4), pp.937-961.
