Name: Anti-DARPP-32 Antibody
Brand: PhosphoSolutions
SKU: 374-DARPP
Price: 415.0 USD
Availability: InStock

Our Anti-DARPP-32 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat DARPP-32 and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

PPP1R1B

Molecular Weight

32 kDa

Go to Full Product Details

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~32 kDa DARPP protein.

Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32(cat. 374-DARPP, green, 1:250) and Tuj1(red). Cells and photo courtesy of QBM CellScience.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-DARPP-32 Antibody

Product Details

Target

DARPP-32

Target Description

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

PPP1R1B

Molecular Weight

32 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus

Antibody Registry ID

AB_2492067

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:250

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~32 kDa DARPP-32 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DARPP32 antibody, DARPP-32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody, Dopamine and cAMP regulated phosphoprotein DARPP32 antibody, Dopamine- and cAMP-regulated neuronal phosphoprotein antibody, FLJ20940 antibody, IPPD antibody, Neuronal phosphoprotein DARPP 32 antibody, PPP1R1B antibody, PPR1B_HUMAN antibody, Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody, Protein phosphatase 1 regulatory inhibitor subunit 1B antibody, Protein phosphatase 1 regulatory subunit 1B antibody

UniProt Details

UniProt (Human): Q9UD71
UniProt (Immunogen Species): Q6J4I0

NCBI Gene ID

360616

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
26657176	not listed	Bonito-Olivia, A., et al. 2016. Involvement of the striatal medium spiny neurons of the direct pathway in the motor stimulant effects of phencyclidine. International Journal of Neuropsychopharmacology, 19(6).
18234897	1:10,000	Niculescu, M., et al. 2008. Trk: A Neuromodulator of Age-Specific Behavioral and Neurochemical Responses to Cocaine in Mice. J. Neurosci., Jan 2008; 28: 1198 - 1207.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-DARPP-32 Antibody

Our Anti-DARPP-32 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat DARPP-32 and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.