Our Anti-DARPP-32 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat DARPP-32 and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32(cat. 374-DARPP, green, 1:250) and Tuj1(red). Cells and photo courtesy of QBM CellScience.
Bulk Order Anti-DARPP-32 Antibody
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).
Antigen Affinity Purified from Pooled Serum
Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).
Bonito-Olivia, A., et al. 2016. Involvement of the striatal medium spiny neurons of the direct pathway in the motor stimulant effects of phencyclidine. International Journal of Neuropsychopharmacology, 19(6).