Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody (M356)

Name: Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CM3561
Price: 340.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M356

Applications

ICC, WB

Host Species

Mouse

Format

Protein A Purified

Gene Name

CTNND1

Molecular Weight

110

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Immunocytochemical labeling of δ1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), or δ1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.

Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2), anti-δ1-Catenin (Tyr-96) (lanes 3 & 4), anti-δ1-Catenin (Tyr-228) (lanes 5 & 6), anti-δ1-Catenin (Tyr-280) (lanes 7 & 8) or anti-δ1-Catenin (Tyr-302) (lanes 9 & 10).

Volume: 100 µL

Variant

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$340

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Product Details

Target

δ1-Catenin (Tyr-228)

Target Description

Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M356

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

CTNND1

Molecular Weight

110

Antigen

Clone M356 was generated from a phospho-δ1-Catenin (Tyr-228) synthetic peptide containing amino acid residues around tyrosine 228 of human δ1-Catenin. This peptide sequence is highly conserved in rat and mouse δ1-Catenin.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 110 kDa* protein corresponding to the molecular mass of δ1-Catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells treated with pervanadate. This antibody does not detect this band after alkaline phosphatase treatment.

Post Translational Modification Type

GOmAb/Phosphorylated

Post Translational Modification Residue

Tyr-228

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

pp120 Src substrate, p120

UniProt Details

UniProt (Human): O60716
UniProt (Immunogen Species): O60716

Product Specific Protocols

Western Blot Protocol: Download

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Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody (M356)

Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody
SKU: CM3561

Bulk Order Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody

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Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody (M356)

Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody SKU: CM3561

Bulk Order Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody

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Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody
SKU: CM3561