Name: Anti-CtIP (Ser327) Antibody
Brand: PhosphoSolutions
SKU: p1012-327
Price: 415.0 USD
Availability: InStock

Our Anti-CtIP (Ser327) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human CtIP (Ser327) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

RBBP8

Molecular Weight

100 kDa

Go to Full Product Details

Western blot of human T47D cell lysate showing specific immunolabeling of the ~100 kDa CtIP phosphorylated at Ser327 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-CtIP (Ser327) Antibody

Product Details

Target

CtIP (Ser327)

Target Description

CtIP, C-terminal binding protein-interacting protein, is a DNA endonuclease activated by double stranded breaks (DSBs). DSB repairs can be performed by either one of two mechanisms; non-homologous end joining (NHEJ) or homologous recombination (HR). NHEJ is the predominant DSB repair pathway throughout the entire cell cycle, most importantly in the G1 phase (Rothkamm et al, 2003); while HR is important for repairing DSBs in S and G2 phases (Beucher et al, 2009). CtIP controls DSB resection; an event that only occurs in HR during G2-phase. Phosphorylation of Thr-847 dictates the resection efficiency (Huertas et al, 2008). Furthermore, it has been found that DSBs undergo resection and repair in G1-phase cells via a process requiring Plk3 phosphorylation of CtIP at Ser-327 and Thr-847 (Barton et al, 2014). Several additional phosphorylation sites within CtIP have been identified, but their significance in the repair of DNA have yet to be determined.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

RBBP8

Molecular Weight

100 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 of human CtIP, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human

Expected Reactivity

Non-Human Primate

Antibody Registry ID

AB_2651148

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~100 kDa CtIP protein phosphorylated at Ser327. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser327

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

COM1 antibody, COM1_HUMAN antibody, CtBP interacting protein antibody, CtBP-interacting protein antibody, CtIP antibody, DNA endonuclease RBBP8 antibody, JWDS antibody, RB binding protein 8 endonuclease antibody, RBBP-8 antibody, RBBP8 antibody, Retinoblastoma-binding protein 8 antibody, Retinoblastoma-interacting protein and myosin-like antibody, Rim antibody, SAE2 antibody, SCKL2 antibody, Sporulation in the absence of SPO11 protein 2 homolog antibody

UniProt Details

UniProt (Human): Q99708
UniProt (Immunogen Species): Q99708

NCBI Gene ID

5932

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
33097710	1:2000	Ming, G., et al. 2020. USP52 regulates DNA end resection and chemosensitivity through removing inhibitory ubiquitination from CtIP. Nature Communications, 11(1), pp.1-13.
25267294	not listed	Barton, O., et al. 2014. Polo-like kinase 3 regulates CtIP during DNA double-strand break repair in G1. J Cell Biol, 206(7), pp.877-894.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-CtIP (Ser327) Antibody

Our Anti-CtIP (Ser327) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human CtIP (Ser327) and is antigen affinity purified from pooled serum. It is great for use in WB.