Our Anti-CtIP (Ser326) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human CtIP (Ser326) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of human T47D cell lysate showing specific immunolabeling of the ~100 kDa CtIP phosphorylated at Ser326 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Anti-CtIP (Ser326) Antibody
CtIP, C-terminal binding protein-interacting protein, is a DNA endonuclease activated by double stranded breaks (DSBs). DSB repairs can be performed by either one of two mechanisms; non-homologous end joining (NHEJ) or homologous recombination (HR). NHEJ is the predominant DSB repair pathway throughout the entire cell cycle, most importantly in the G1 phase (Rothkamm et al, 2003); while HR is important for repairing DSBs in S and G2 phases (Beucher et al, 2009). CtIP controls DSB resection; an event that only occurs in HR during G2-phase. Phosphorylation of Thr-847 dictates the resection efficiency (Huertas et al, 2008). Furthermore, it has been found that DSBs undergo resection and repair in G1-phase cells via a process requiring Plk3 phosphorylation of CtIP at Ser-327 and Thr-847 (Barton et al, 2014). Several additional phosphorylation sites within CtIP have been identified, but their significance in the repair of DNA have yet to be determined.
Antigen Affinity Purified from Pooled Serum
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser326 of human CtIP, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Specific for endogenous levels of the ~100 kDa CtIP protein phosphorylated at Ser326. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
COM1 antibody, COM1_HUMAN antibody, CtBP interacting protein antibody, CtBP-interacting protein antibody, CtIP antibody, DNA endonuclease RBBP8 antibody, JWDS antibody, RB binding protein 8 endonuclease antibody, RBBP-8 antibody, RBBP8 antibody, Retinoblastoma-binding protein 8 antibody, Retinoblastoma-interacting protein and myosin-like antibody, Rim antibody, SAE2 antibody, SCKL2 antibody, Sporulation in the absence of SPO11 protein 2 homolog antibody
Rothkamm, K., Krüger, I., Thompson, L.H. and Löbrich, M., 2003. Pathways of DNA double-strand break repair during the mammalian cell cycle. Molecular and cellular biology, 23(16), pp.5706-5715. PMID: 12897142
Beucher, A., Birraux, J., Tchouandong, L., Barton, O., Shibata, A., Conrad, S., Goodarzi, A.A., Krempler, A., Jeggo, P.A. and Löbrich, M., 2009. ATM and Artemis promote homologous recombination of radiation‐induced DNA double‐strand breaks in G2. The EMBO journal, 28(21), pp.3413-3427. PMID: 19779458
Huertas, P. and Jackson, S.P., 2009. Human CtIP mediates cell cycle control of DNA end resection and double strand break repair. Journal of Biological Chemistry, 284(14), pp.9558-9565. PMID: 19202191
Barton, O., Naumann, S.C., Diemer-Biehs, R., Künzel, J., Steinlage, M., Conrad, S., Makharashvili, N., Wang, J., Feng, L., Lopez, B.S. and Paull, T.T., 2014. Polo-like kinase 3 regulates CtIP during DNA double-strand break repair in G1. J Cell Biol, 206(7), pp.877-894. PMID: 25267294
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