Anti-CRMP2 (Thr-555), Phosphospecific Antibody (M539)

Name: Anti-CRMP2 (Thr-555), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CM5391
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

1 Citation

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M539

Applications

Host Species

Mouse

Format

Antigen Affinity Purified

Gene Name

DPYSL2

Molecular Weight

Go to Full Product Details

Western blot of rat PC12 cells stimulated with calyculin A (100 nM) for 30 min (lanes 1-4). Then the blot was treated with lambda phosphatase to dephosphorylate CRMP2 (lanes 2 & 4). The blot was probed with rabbit polyclonal anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or mouse monoclonal anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).

Volume: 100 µL

Variant

Price:

$309

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Bulk Order Anti-CRMP2 (Thr-555), Phosphospecific Antibody

Product Details

Target

CRMP2 (Thr-555)

Target Description

CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518,Thr-514, and Thr-509), and a priming site (Ser-522). These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.

Format

Antigen Affinity Purified

Clonality

Monoclonal

Clone

M539

Isotype

IgG1

Applications

Host Species

Mouse

Gene Name

DPYSL2

Molecular Weight

Antigen

Clone M539 was generated from a phospho-CRMP2 (Thr-555) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-555 in human CRMP2. This sequence is conserved in rat and mouse CRMP2 and the phospho-site is not conserved in other CRMP family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

The antibody detects a 70 kDa* protein corresponding to CRMP2 on immunoblots of rat PC12 or mouse C2C12 cells treated with calyculin A. This reactivity is not observed after lambda phosphatase dephosphorylation of Thr-555.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr-555

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DRP-2, Toad-64, CRMP-62

UniProt Details

UniProt (Human): Q16555
UniProt (Immunogen Species): Q16555

Product Specific References for Applications and Species

Western Blot: Rat

Western Blot: Rat
PMID	Dilution	Publication
35334412	1:1000	Li, Y, et al. 2022. CRMP2 Modulates Mossy Fiber Sprouting in Dentate Gyrus of Pilocarpine Induced Rat Model of Epilepsy. Biochemical and Biophysical Research Communications, 141-147.

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Anti-CRMP2 (Thr-555), Phosphospecific Antibody (M539)

Anti-CRMP2 (Thr-555), Phosphospecific Antibody
SKU: CM5391

Bulk Order Anti-CRMP2 (Thr-555), Phosphospecific Antibody

Product Details

Product Specific References for Applications and Species

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Anti-CRMP2 (Thr-555), Phosphospecific Antibody (M539)

Anti-CRMP2 (Thr-555), Phosphospecific Antibody SKU: CM5391

Bulk Order Anti-CRMP2 (Thr-555), Phosphospecific Antibody

Product Details

Product Specific References for Applications and Species

Anti-CRMP2 (Thr-555), Phosphospecific Antibody
SKU: CM5391