Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).
Anti-CRMP2 (Ser-522), Phosphospecific Antibody
SKU: CP2191
Bulk Order Anti-CRMP2 (Ser-522), Phosphospecific Antibody
Product Details
CRMP2 (Ser-522)
CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518,Thr-514, and Thr-509), and a priming site (Ser-522). These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
DPYSL2
70
Phospho-CRMP2 (Ser-522) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-522 in human CRMP2. This sequence is conserved in rat and mouse CRMP2, and has homology to the conserved site in CRMP1 and CRMP4, but is not conserved in CRMP3 and CRMP5.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
Unconjugated
The antibody detects a 70 kDa* protein corresponding to the molecular mass of CRMP2 on SDS-PAGE immunoblots of human mouse brain, and this band is removed after lambda phosphatase treatment. In addition, this reactivity can be specifically blocked using phospho-CRMP2 (Ser-522) peptide (CX2195).
Phosphorylated
Ser-522
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
