Anti-CRMP2 (Ser-522), Phosphospecific Antibody

Name: Anti-CRMP2 (Ser-522), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CP2191
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

24 Citations

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

DPYSL2

Molecular Weight

Go to Full Product Details

Immunocytochemical labeling of CRMP2 phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-CRMP2 (Ser-522) (CP2191) antibody in the absence (Left) or presence (Right) of blocking peptide (CX2195). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).

Volume: 100 µL

Variant

Price:

$309

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Product Details

Target

CRMP2 (Ser-522)

Target Description

CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518,Thr-514, and Thr-509), and a priming site (Ser-522). These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

DPYSL2

Molecular Weight

Antigen

Phospho-CRMP2 (Ser-522) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-522 in human CRMP2. This sequence is conserved in rat and mouse CRMP2, and has homology to the conserved site in CRMP1 and CRMP4, but is not conserved in CRMP3 and CRMP5.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 70 kDa* protein corresponding to the molecular mass of CRMP2 on SDS-PAGE immunoblots of human mouse brain, and this band is removed after lambda phosphatase treatment. In addition, this reactivity can be specifically blocked using phospho-CRMP2 (Ser-522) peptide (CX2195).

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-522

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DRP-2, Toad-64, CRMP-62

UniProt Details

UniProt (Human): Q16555
UniProt (Immunogen Species): Q16555

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Mouse | Rat
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
33401710	not listed	Paik, S, et al. 2021. Somatostatin Ameliorates β-Amyloid-Induced Cytotoxicity via the Regulation of CRMP2 Phosphorylation and Calcium Homeostasis in SH-SY5Y Cells. Biomedicines, 27.

Immunocytochemistry: Rat
PMID	Dilution	Publication
28809766	not listed	Moutal, A, et al. 2017. CRISPR/Cas9 editing of Nf1 gene identifies CRMP2 as a therapeutic target in neurofibromatosis type 1-related pain that is reversed by (S)-Lacosamide. Pain, 2301-2319.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
34297816	1:200	Yamazaki, Y, et al. 2022. Requirement of CRMP2 Phosphorylation in Neuronal Migration of Developing Mouse Cerebral Cortex and Hippocampus and Redundant Roles of CRMP1 and …. Cerebral cortex, 520-527.
32973242	1:1000	Nishijima, E, et al. 2020. Topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury. Scientific reports, 15709.
32156571	1:200	Yamazaki, Y, et al. 2020. Phosphorylation of CRMP2 is required for migration and positioning of Purkinje cells: Redundant roles of CRMP1 and CRMP4. Brain research, 146762.
30375127	1:200	Togashi, K, et al. 2019. Genetic suppression of collapsin response mediator protein 2 phosphorylation improves outcome in methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson’s model mice. Genes to cells : devoted to molecular & cellular mechanisms, 31-40.
28660485	not listed	Moutal, A, et al. 2018. CRMP2 Phosphorylation Drives Glioblastoma Cell Proliferation. Molecular neurobiology, 4403-4416.

Immunohistochemistry: Rat
PMID	Dilution	Publication
32571373	not listed	Moutal, A, et al. 2020. Differential expression of Cdk5-phosphorylated CRMP2 following a spared nerve injury. Molecular brain, 97.
27917413	not listed	Moutal, A, et al. 2016. Efficacy of ( S)-Lacosamide in preclinical models of cephalic pain. Pain reports, e565.

Western Blot: Human
PMID	Dilution	Publication
33401710	1:1000	Paik, S, et al. 2021. Somatostatin Ameliorates β-Amyloid-Induced Cytotoxicity via the Regulation of CRMP2 Phosphorylation and Calcium Homeostasis in SH-SY5Y Cells. Biomedicines, 17.
30816227	not listed	Duciel, L, et al. 2019. Protein tyrosine phosphatase 4A3 (PTP4A3/PRL-3) promotes the aggressiveness of human uveal melanoma through dephosphorylation of CRMP2. Scientific reports, 2990.
29926835	1:2000	Mokhtar, S, et al. 2018. Amyloid-beta-dependent phosphorylation of collapsin response mediator protein-2 dissociates kinesin in Alzheimer's disease. Neural regeneration research, 1066-1080.

Western Blot: Mouse
PMID	Dilution	Publication
37174687	1:1500	Brustovetsky, T, et al. 2023. CRMP2 Participates in Regulating Mitochondrial Morphology and Motility in Alzheimer's Disease. Cells, 1287.
35589915	1:1000	Yazawa, A, et al. 2022. Effects of Lanthionine Ketimine-5-Ethyl Ester on the α-Synucleinopathy Mouse Model. Neurochemical Research, 2373-2382.
34831395	1:1500	Brustovestsky, T, et al. 2021. Involvement of CRMP2 in Regulation of Mitochondrial Morphology and Motility in Huntington's Disease. Cells, 3172.
34685760	1:1500	Brustovestsky, T, et al. 2021. CRMP2 Is Involved in Regulation of Mitochondrial Morphology and Motility in Neurons. Cells, 2781.
31795726	not listed	Moutal, A, et al. 2019. Neuronal Conditional Knockout of Collapsin Response Mediator Protein 2 Ameliorates Disease Severity in a Mouse Model of Multiple Sclerosis. ASN Neuro, 44943.
28648892	not listed	Liu, R, et al. 2017. ASCIZ/ATMIN is dispensable for ATM signaling in response to replication stress. DNA repair, 29-34.

Western Blot: Rat
PMID	Dilution	Publication
35334412	1:1000	Li, Y, et al. 2022. CRMP2 Modulates Mossy Fiber Sprouting in Dentate Gyrus of Pilocarpine Induced Rat Model of Epilepsy. Biochemical and Biophysical Research Communications, 141-147.
36564853	1:1000	Jiang, YP, et al. 2022. Neuronal CRMP2 phosphorylation inhibition by the flavonoid, naringenin, contributes to the reversal of spinal sensitization and arthritic pain improvement. Arthritis research & therapy, 277.
31680630	not listed	Moutal, A, et al. 2019. Evaluation of edonerpic maleate as a CRMP2 inhibitor for pain relief. Channels (Austin, Tex.), 498-504.
31080913	not listed	Moutal, A, et al. 2019. Cdk5-mediated CRMP2 phosphorylation is necessary and sufficient for peripheral neuropathic pain. Neurobiology of pain (Cambridge, Mass.), 100022.
28767512	not listed	Moutal, A, et al. 2017. Dissecting the role of the CRMP2-neurofibromin complex on pain behaviors. Pain, 2203-2221.
26967696	not listed	Moutal, A, et al. 2016. (S)-lacosamide inhibition of CRMP2 phosphorylation reduces postoperative and neuropathic pain behaviors through distinct classes of sensory neurons identified by constellation pharmacology. Pain reports, 1448-1463.

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Anti-CRMP2 (Ser-522), Phosphospecific Antibody