Anti-CREB (N-terminal region) Antibody

Name: Anti-CREB (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CP4141
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

CREB1

Molecular Weight

Go to Full Product Details

Immunocytochemical labeling of CREB in A431 that were fixed in paraformaldehyde and permeabilized using NP-40. The cells were labeled with rabbit polyclonal CREB (N-terminal region). The antibody was detected using goat anti-rabbit DyLight® 594.

Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).

Volume: 100 µL

Variant

Price:

$255

Quantity:

Bulk Order Anti-CREB (N-terminal region) Antibody

Product Details

Target

CREB (N-terminal region)

Target Description

CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles in cell survival and proliferation. CREB is expressed in various tissues, and has important gene-regulating roles in the nervous system. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases. CREB is phosphorylated at other sites in response to calcium influx and DNA damage. The DNA-damage responsive nuclear kinase, HIPK2, can phosphorylate Ser-271 but not Ser-133 in CREB, and this phosphorylation activates CREB transactivation function. Mutation of Ser-271 to Glu-271 potentiates the CREB transactivation function. Thus, phosphorylation of Ser-271 may be the mode of activation for CREB-dependent transcription in response to genotoxic stress.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IP, WB

Host Species

Rabbit

Gene Name

CREB1

Molecular Weight

Antigen

CREB synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminal region of human CREB. This sequence is well conserved in rat, mouse, and chicken CREB, and has low homology to other CREB family members.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 43 kDa* protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human A431, Jurkat, HeLa, and rat PC12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Cyclic AMP, CREB

UniProt Details

UniProt (Human): P16220
UniProt (Immunogen Species): P16220

Product Specific Protocols

Western Blot Protocol: Download

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Anti-CREB (N-terminal region) Antibody