Name: Anti-Collagen 1, alpha 1 telopeptide Antibody
Brand: PhosphoSolutions
SKU: 322-COLT
Price: 415.0 USD
Availability: InStock

Our Anti-Collagen 1, alpha 1 telopeptide primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, rat, and sheep Collagen 1, alpha 1 telopeptide and is antigen affinity purified. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat, Sheep

Expected Reactivity

Bovine, Canine, Chicken, Feline, Finch, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Vole, Xenopus

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

COL1A1

Molecular Weight

140 kDa

Go to Full Product Details

Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (cat. 322-COLT, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.

Immunofluorescence of a transversal section from mouse spinal cord labeling fibers of collagen (cat. : 322-COLT, red, 1:200) from the tissue scaffold. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.

Western blot of rat lung lysate showing specific immunolabeling of the ~140 kDa collagen protein.

Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (cat. 322-COLT, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Collagen 1, alpha 1 telopeptide Antibody

Product Details

Target

Collagen 1, alpha 1 telopeptide

Target Description

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the most abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the first 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

COL1A1

Molecular Weight

140 kDa

Antigen

Synthetic peptide corresponding to amino acid residues specific to the C-terminal region of human collagen I alpha 1 telopeptide, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat, Sheep

Expected Reactivity

Bovine, Canine, Chicken, Feline, Finch, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Vole, Xenopus

Antibody Registry ID

AB_2492061

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Affinity purified rabbit serum.

Buffer

PBS

Dilution Ranges

WB: 1:1000
IHC: 1:100-1:200

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~140 kDa collagen I alpha-1 telopeptide portion of the collagen I alpha-1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0) / 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Alpha 1 type I collagen antibody, Alpha 2 type I collagen antibody, alpha 2 type I procollagen antibody, alpha 2(I) procollagen antibody, alpha 2(I)-collagen antibody, Alpha-1 type I collagen antibody, alpha1(I) procollagen antibody, CO1A1_HUMAN antibody, COL1A1 antibody, COL1A2 antibody, collagen alpha 1 chain type I antibody, Collagen alpha-1(I) chain antibody, collagen alpha-1(I) chain preproprotein antibody, Collagen I alpha 1 polypeptide antibody, Collagen I alpha 2 polypeptide antibody, collagen of skin tendon and bone, alpha-1 chain antibody, collagen of skin tendon and bone alpha-2 chain antibody, Collagen type I alpha 1 antibody, Collagen type I alpha 2 antibody, EDSC antibody, OI1 antibody, OI2 antibody, OI3 antibody, OI4 antibody, pro-alpha-1 collagen type 1 antibody, type I proalpha 1 antibody, type I procollagen alpha 1 chain antibody, Type I procollagen antibody

UniProt Details

UniProt (Human): P02452
UniProt (Immunogen Species): P02452

NCBI Gene ID

1277

Product Specific References for Applications and Species

Immunohistochemistry: Human | Mouse

Immunohistochemistry: Human
PMID	Dilution	Publication
23004679	not listed	Bains, S.N., et al. 2012. Loss of caveolin-1 from bronchial epithelial cells and monocytes in human subjects with asthma. Allergy. 2012 Dec;67(12): 1601-4.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
33951398	not listed	Schiffers, C., et al. 2021. Downregulation of DUOX1 function contributes to aging-related impairment of innate airway injury responses and accelerated senile emphysema. Journal of Physiology-Lung Cellular and Molecular Physiology, 321(1).
30117615	1:500	Gatto, R.G., et al. 2018. In vivo diffusion MRI detects early spinal cord axonal pathology in a mouse model of amyotrophic lateral sclerosis. NMR in Biomedicine, Aug;31(8):e3954.
22546693	not listed	Wessels, A., et al. 2012. Epicardially derived fibroblasts preferentially contribute to the parietal leaflets of the atrioventricular valves in the murine heart. Dev Biol. 2012 Jun 15;366(2): 111-24.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Collagen 1, alpha 1 telopeptide Antibody

Our Anti-Collagen 1, alpha 1 telopeptide primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, rat, and sheep Collagen 1, alpha 1 telopeptide and is antigen affinity purified. It is great for use in WB, IHC.