Western blot of rat hippocampal lysate showing specific immunolabeling of the ~35 kDa clavesin protein.
Clavesin (clathrin vesicle associated Sec14 protein) is a novel neuron specific protein that has recently been identified and shown to be required for normal morphology of late endosomes and/or lysosomes as lentiviral-mediated knockdown of clavesin in hippocampal neurons causes lysosomal defects (Katoh et al., 2009). Additionally, upregulation of clavesin in human hepatocellular carcinoma has recently been demonstrated thus making it a useful marker for this disease state (Zhao et al., 2008).
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat Clavesin 1, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from serum by affinity purification using a column to which the peptide antigen was coupled.
PBS + 50% glycerol
WB Brain: 1:1000
Specific for endogenous levels of the ~35 kDa clavesin 1/2 protein doublet. Isoform-specific knock down in cultured hippocampal neurons indicates that the lower and upper bands are clavesin 1 and 2, respectively.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
4 antibody, C6orf212 antibody, C6orf213 antibody, Clavesin 1 antibody, Clavesin 2 antibody, CRALBPL antibody, FLJ37248 antibody, MGC120572 antibody, MGC34646 antibody, OTTHUMP00000017122 antibody, Retinaldehyde binding protein 1 like 1 antibody, Retinaldehyde binding protein 1 like 2 antibody, RLBP1L1 antibody, RLBP1L2 antibody