Name: Anti-CK1Mt (Tyr153) Antibody
Brand: PhosphoSolutions
SKU: p145-153
Price: 415.0 USD
Availability: InStock

Our Anti-CK1Mt (Tyr153) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse CK1Mt (Tyr153) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse

Expected Reactivity

Bovine, Canine, Guinea Pig, Hamster, Non-Human Primate, Rabbit, Rat, Sheep

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

CKMT1A

Molecular Weight

46 kDa

Go to Full Product Details

Western blot of mouse mitochondria from whole brain lysate showing specific immunolabeling of the ~46 kDa CK1Mt protein phosphorylated at Tyr153 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is greatly decreased by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-CK1Mt (Tyr153) Antibody

Product Details

Target

CK1Mt (Tyr153)

Target Description

Creatine kinase (CK1) plays an important role transferring phosphate groups from phosphocreatine to ADP in the cytosol of tissues having high, fluctuating energy demands like skeletal muscle, heart, and brain. Mitochondrial CK1, CK1Mt, has two isoforms, sarcomeric CK1Mt and ubiquitous CK1Mt. CK1Mt is localized between the MIM (mitochondrial inner membrane) and MOM (mitochondrial outer membrane) and bound to the cardiolipin-rich inner leaflet (Muller et al., 1985) and along the cristae membranes (Wegmann et al., 1991). CK1Mt exists in two forms; a homo dimer and an octamer consisting of four homodimers. The CK1Mt octamer forms permanent contact sites and maintains a complex structure including porin and ANT (adenine nucleotide translocase) within MIM and MOM (Speer et al, 2005). There have been several phospho-serine, threonine, and tyrosine sites identified within CK1Mt, the role of each one has yet to be determined.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

CKMT1A

Molecular Weight

46 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr153 of human CK1Mt, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse

Expected Reactivity

Bovine, Canine, Guinea Pig, Hamster, Non-Human Primate, Rabbit, Rat, Sheep

Antibody Registry ID

AB_2632396

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~46 kDa CK1Mt protein phosphorylated at Tyr153. Immunolabeling of CK1Mt is greatly decreased by lambda-phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr153

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Acidic type mitochondrial creatine kinase antibody, CKMT antibody, CKMT1 antibody, CKMT1A antibody, CKMT1B antibody, Creatine kinase mitochondrial 1 ubiquitous antibody, Creatine kinase U type mitochondrial antibody, Creatine kinase ubiquitous mitochondrial antibody, Creatine kinase mitochondrial 1 (ubiquitous) antibody, Creatine kinase mitochondrial 1A antibody, Creatine kinase mitochondrial 1B antibody, Mia CK antibody, U MtCK antibody, Ubiquitous mitochondrial creatine kinase antibody, UMTCK antibody

UniProt Details

UniProt (Human): P12532
UniProt (Immunogen Species): P12532

NCBI Gene ID

1159

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
30174304	not listed	Kurmi, K., et al. 2018. Tyrosine phosphorylation of mitochondrial creatine kinase 1 enhances a druggable tumor energy shuttle pathway. Cell metabolism, 28(6), pp.833-847.

Western Blot: Mouse
PMID	Dilution	Publication
30174304	not listed	Kurmi, K., et al. 2018. Tyrosine phosphorylation of mitochondrial creatine kinase 1 enhances a druggable tumor energy shuttle pathway. Cell metabolism, 28(6), pp.833-847.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-CK1Mt (Tyr153) Antibody

Our Anti-CK1Mt (Tyr153) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse CK1Mt (Tyr153) and is antigen affinity purified from pooled serum. It is great for use in WB.