Name: Anti-Che-1 (AATF) (Ser477) Antibody
Brand: PhosphoSolutions
SKU: p142-477
Price: 415.0 USD
Availability: InStock

Our Anti-Che-1 (AATF) (Ser477) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Che-1 (AATF) (Ser477) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Bovine, Canine, Mouse, Non-Human Primate, Rat, Sheep, Xenopus

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

AATF

Molecular Weight

66 kDa

Go to Full Product Details

Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser477 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Che-1 (AATF) (Ser477) Antibody

Product Details

Target

Che-1 (AATF) (Ser477)

Target Description

Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser-477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

AATF

Molecular Weight

66 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 of human Che-1, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human

Expected Reactivity

Bovine, Canine, Mouse, Non-Human Primate, Rat, Sheep, Xenopus

Antibody Registry ID

AB_2492057

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~66 kDa Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser477

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

AATF antibody, AATF_HUMAN antibody, Apoptosis antagonizing transcription factor antibody, Apoptosis-antagonizing transcription factor antibody, BFR2 antibody, CHE 1 antibody, CHE1 antibody, DED antibody, Protein AATF antibody, Rb binding protein Che 1 antibody, Rb-binding protein Che-1 antibody

UniProt Details

UniProt (Human): Q9NY61
UniProt (Immunogen Species): Q9NY61

NCBI Gene ID

26574

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
27012205	not listed	Liu, M., et al. 2016. Che-1 gene silencing induces osteosarcoma cell apoptosis by inhibiting mutant p53 expression. Biochemical and biophysical research communications, 473(1), pp.168-173.
25996291	not listed	Desantis, A., et al. 2015. Che-1 modulates the decision between cell cycle arrest and apoptosis by its binding to p53. Cell death & disease, 6(5), p.e1764.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Che-1 (AATF) (Ser477) Antibody

Our Anti-Che-1 (AATF) (Ser477) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Che-1 (AATF) (Ser477) and is antigen affinity purified from pooled serum. It is great for use in WB.