Our Anti-Che-1 (AATF) (Ser477) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Che-1 (AATF) (Ser477) and is antigen affinity purified from pooled serum. It is great for use in WB.
Primary Antibody
Human
Bovine, Canine, Mouse, Non-Human Primate, Rat, Sheep, Xenopus
WB
Rabbit
Antigen Affinity Purified from Pooled Serum
AATF
66 kDa

Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser477 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Product Specific References for Applications and Species
- Western Blot: Human
Western Blot: Human | ||
PMID | Dilution | Publication |
27012205 | not listed | Liu, M., et al. 2016. Che-1 gene silencing induces osteosarcoma cell apoptosis by inhibiting mutant p53 expression. Biochemical and biophysical research communications, 473(1), pp.168-173. |
25996291 | not listed | Desantis, A., et al. 2015. Che-1 modulates the decision between cell cycle arrest and apoptosis by its binding to p53. Cell death & disease, 6(5), p.e1764. |
Product Specific Protocols
- Western Blot Protocol: Download