Anti-Cdk1 (N-terminal region) Antibody (M226)

Name: Anti-Cdk1 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CM2261
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M226

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Format

Protein A Purified

Gene Name

CDK1

Molecular Weight

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Western blot analysis of human SYF fibroblasts before (lanes 1 & 3) and after (lanes 2 & 4) treatment with alkaline phosphatase. The blots were probed with anti-Cdk1 (N-terminal region) antibody (lanes 1 & 2) or anti-Cdk1 (Tyr-15) phospho-specific antibody (lanes 3 & 4).

Volume: 100 µL

Variant

Price:

$255

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Bulk Order Anti-Cdk1 (N-terminal region) Antibody

Product Details

Target

Cdk1 (N-terminal region)

Target Description

Cyclin-dependent kinases (Cdks) are a family of serine/threonine kinases that require association with regulatory subunits known as cyclins for activation. In addition, post-translational phosphorylation and dephosphorylation events regulate Cdk activity. Phosphorylation of Thr-160 in the T loop by Cdk-activating kinase (CAK) is an obligatory step in kinase activation. By contrast, phosphorylation of the Thr-14 and Tyr-15 residues by the Wee1 family of dual specificity kinases is inhibitory for the Cdks, and dephosphorylation of these residues by the Cdc25 family of phosphatases coincides with Cdk activation. Alternatively, Cdk5 appears to require different mechanisms for activation. This Cdk is activated through association with specific activators, including p35, p39, and p67. Cdk5 is primarily activated in neuronal cells, and only c-Abl kinase, rather than Wee family members, have been shown to phosphorylate Tyr-15 to regulate its activity.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M226

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

CDK1

Molecular Weight

Antigen

Clone M226 was generated from a recombinant human Cdk1 protein that included amino acids residues in the N-terminal region. This sequence is conserved in human and rat Cdk1, and has low homology to other Cdk family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 34 kDa* band corresponding to Cdk1 on SDS-PAGE immunoblots of human SYF and HeLa cells. The antibody does not detect other Cdk family members, such as Cdk2 and Cdk5.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Cdc2

UniProt Details

UniProt (Human): P06493
UniProt (Immunogen Species): P06493

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Cdk1 (N-terminal region) Antibody (M226)

Anti-Cdk1 (N-terminal region) Antibody
SKU: CM2261

Bulk Order Anti-Cdk1 (N-terminal region) Antibody

Product Details

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Anti-Cdk1 (N-terminal region) Antibody (M226)

Anti-Cdk1 (N-terminal region) Antibody SKU: CM2261

Bulk Order Anti-Cdk1 (N-terminal region) Antibody

Product Details

Product Specific Protocols

Anti-Cdk1 (N-terminal region) Antibody
SKU: CM2261