Anti-CD73/NT5E Antibody (M064)

Name: Anti-CD73/NT5E Antibody
Brand: PhosphoSolutions
SKU: CM0641
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human

Clone

M064

Applications

IP, WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

CD73

Molecular Weight

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Western blot of native lysates including human CD73 recombinant protein (lane 1), NCI-H1915 lung carcinoma (lane 2), MeWo melanoma (lane 3), NCI-H1299 lung carcinoma (lane 4), and MDA-MB-231 breast carcinoma (lane 5). The blot was probed with mouse monoclonal anti-CD73 (CM0641) at 1:500.

Volume: 100 µL

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$309

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Product Details

Target

CD73/NT5E

Target Description

CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer. CD73 has been implicated as a prognostic indicator and therapeutic target in cancers. CD73 may act as an immune checkpoint inhibitor to natural killer cells in tumors, and this activity correlates with tumor invasiveness, metastasis, and poor outcome in cancer patients.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M064

Isotype

IgG1

Applications

IP, WB

Host Species

Mouse

Gene Name

CD73

Molecular Weight

Antigen

Clone (M064) was generated from a proprietary antigen related to the mature form of CD73 expressed in the NCI-H1299 lung cancer cell line.

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
IP: 1:50

Conjugate

Unconjugated

Specificity

Clone M064 mouse monoclonal antibody detects a 65 kDa* protein on SDSPAGE "Native" immunoblots of human H1299, H1915, and MDA-MB-231 carcinomas. This antibody weakly detects denatured CD73. The antibody works for native western blot, immunoprecipitation, and ELISA.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

5'-nucleotidase, NT5E, NTE, CD73, NT5

UniProt Details

UniProt (Human): P21589
UniProt (Immunogen Species): P21589

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Anti-CD73/NT5E Antibody (M064)

Anti-CD73/NT5E Antibody
SKU: CM0641

Bulk Order Anti-CD73/NT5E Antibody

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