Anti-c-FOS Antibody (2H2)

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Our Anti-c-FOS primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat c-FOS and is Protein G purified. It is great for use in WB, IHC, ICC.

Primary Antibody
Bovine, Human, Mouse, Rat
2H2
ICC, IHC, WB
Mouse
Protein G Purified
FOS
50 kDa

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.
Immunofluorescence of a section of rat hippocampus showing neurons labeled with c-FOS (cat. 309-cFOS, 1:1000, red) counterstained with FOX3(NeuN) (green), and nuclear staining with DAPI. When both c-FOS and FOX3 are expressed the label appears orange.
Immunostaining of mouse brain labeling c-Fos protein in green(cat. 309-cFOS, 1:1000) and DNA with DAPI. Photo courtesy of Adam Almeida, University of Colorado.
Immunolabeling of a section of rat brain that specifically labels c-FOS protein(cat. 309-cFOS, green, 1:1000). Frozen sections were fixed with 4%PFA for 24 hours. Before performing free floating immunohistochemistry, sections underwent antigen retrieval with sodium citrate (pH6.0) for 30 minutes at 80˚C. Image kindly provided by Angela Gonzalez, Washington State University, Vancouver.
Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.
Immunofluorescence of a section of rat hippocampus showing neurons labeled with c-FOS (cat. 309-cFOS, 1:1000, red) counterstained with FOX3(NeuN) (green), and nuclear staining with DAPI. When both c-FOS and FOX3 are expressed the label appears orange.
Immunostaining of mouse brain labeling c-Fos protein in green(cat. 309-cFOS, 1:1000) and DNA with DAPI. Photo courtesy of Adam Almeida, University of Colorado.
Immunolabeling of a section of rat brain that specifically labels c-FOS protein(cat. 309-cFOS, green, 1:1000). Frozen sections were fixed with 4%PFA for 24 hours. Before performing free floating immunohistochemistry, sections underwent antigen retrieval with sodium citrate (pH6.0) for 30 minutes at 80˚C. Image kindly provided by Angela Gonzalez, Washington State University, Vancouver.
Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).

Anti-c-FOS Antibody
SKU: 309-cFOS

Volume: 100 µL
Price:
$399

Bulk Order Anti-c-FOS Antibody

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PMID Dilution Publication
32572463 1:1000 Yu, T., et al. 2020. Genetic Deletion of TrpV1 and TrpA1 Does not Alter Avoidance of or Patterns of Brainstem Activation to Citric Acid in Mice. Chemical Senses, 45(7), pp.573-579.
Immunohistochemistry: Rat
PMID Dilution Publication
34650409 1:800 Ferraro, S., et al. 2021. In utero Exposure to Valproic-Acid Alters Circadian Organisation and Clock-Gene Expression: Implications for Autism Spectrum Disorders. Frontiers in Behavioral Neuroscience, 15.

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