Anti-c-Cbl (Tyr-700), Phosphospecific Antibody (M161)

Name: Anti-c-Cbl (Tyr-700), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CM1611
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human

Clone

M161

Applications

Host Species

Mouse

Format

Protein A Purified

Gene Name

CBL

Molecular Weight

120

Go to Full Product Details

Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-c-Cbl (CM1591; lanes 1 & 2) or anti-c-Cbl (Tyr-700) (CM1611; lanes 3 & 4).

Volume: 100 µL

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Product Details

Target

c-Cbl (Tyr-700)

Target Description

c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells. c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain and a C3HC4 RING finger motif. c-Cbl is rapidly tyrosine phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol 3 kinase (PI3K), Crk and 14-3-3 proteins. In human cancer tissues, c-Cbl is frequently tyrosine phosphorylated in a tumor-specific manner. Src-family kinases primarily phosphorylate c-Cbl at Tyr-700, Tyr-731, and Tyr-774. These sites of phosphorylation provide docking sites for downstream signaling components, such as Grb-2, PI3K, and Fyn.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M161

Isotype

IgG1

Applications

Host Species

Mouse

Gene Name

CBL

Molecular Weight

120

Antigen

Clone (M161) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 700 in human c-Cbl. This sequence is highly conserved in rat and mouse c-Cbl.

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 120 kDa* protein in human Jurkat cells treated with pervanadate, but not in control cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-700

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

E3 ubiquitin ligase CBL, RING finger 55

UniProt Details

UniProt (Human): P22681
UniProt (Immunogen Species): P22681

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Anti-c-Cbl (Tyr-700), Phosphospecific Antibody (M161)

Anti-c-Cbl (Tyr-700), Phosphospecific Antibody
SKU: CM1611

Bulk Order Anti-c-Cbl (Tyr-700), Phosphospecific Antibody

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Anti-c-Cbl (Tyr-700), Phosphospecific Antibody (M161)

Anti-c-Cbl (Tyr-700), Phosphospecific Antibody SKU: CM1611

Bulk Order Anti-c-Cbl (Tyr-700), Phosphospecific Antibody

Product Details

Anti-c-Cbl (Tyr-700), Phosphospecific Antibody
SKU: CM1611