Anti-c-Abl (C-terminal region) Antibody (M209)

Name: Anti-c-Abl (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: AM2091
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M209

Applications

Host Species

Mouse

Format

Protein G Purified

Gene Name

ABL1

Molecular Weight

145

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Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (AP1271; lanes 3 & 4), or anti-c-Abl (Tyr-245) (AP1251; lanes 5 & 6).

Volume: 100 µL

Variant

Price:

$255

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Bulk Order Anti-c-Abl (C-terminal region) Antibody

Product Details

Target

c-Abl (C-terminal region)

Target Description

The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is widely expressed and is distributed in both the nucleus and the cytoplasm of cells. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion, and stress response. A variety of stimuli activate c-Abl kinase including integrin activation, PDGF stimulation, and binding to proteins, such as c-Jun. Tyrosine phosphorylation is important for the regulation of c-Abl kinase activity. Tyrosine 245 is located in the linker region between the SH2 and catalytic domains. Phosphorylation of Tyr-245 is involved in activation of c-Abl kinase activity. Tyrosine 412 is located in the kinase activation loop of c-Abl, and phosphorylation of this residue is required for kinase activity. Thus, phosphorylation of Tyr-245 and Tyr-412 may be critical for activation of c-Abl in a variety of cell signaling pathways.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M209

Isotype

IgG1

Applications

Host Species

Mouse

Gene Name

ABL1

Molecular Weight

145

Antigen

Clone (M209) was generated from a recombinant protein corresponding to the C-terminal region of human c-Abl.

Species Reactivity

Human, Mouse, Rat

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.2% gelatin and 0.09% NaN3.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody detects a 145 kDa* protein corresponding to c-Abl on SDS-PAGE immunoblots of human K-562 and HL-60 cells, and mouse ANN-1 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

BCR-Abl, p150, Abelson murine leukemia viral oncogene homolog 1

UniProt Details

UniProt (Human): P00519
UniProt (Immunogen Species): P00519

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Anti-c-Abl (C-terminal region) Antibody (M209)

Anti-c-Abl (C-terminal region) Antibody
SKU: AM2091

Bulk Order Anti-c-Abl (C-terminal region) Antibody

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Anti-c-Abl (C-terminal region) Antibody (M209)

Anti-c-Abl (C-terminal region) Antibody SKU: AM2091

Bulk Order Anti-c-Abl (C-terminal region) Antibody

Product Details

Anti-c-Abl (C-terminal region) Antibody
SKU: AM2091