Anti-Blasticidin S Deaminase Antibody

Name: Anti-Blasticidin S Deaminase Antibody
Brand: PhosphoSolutions
SKU: BP1231
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

BSD

Molecular Weight

Go to Full Product Details

Immunocytochemical labeling of Blasticidin S Deaminase (Bsd) in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were labeled with anti-Bsd (BP1231) and detected using appropriate secondary antibody conjugated to Texas Red. (Images provided by Charles Mashburn and Dr. George Smith at the University of Kentucky, Spinal Cord and Brain Injury Research Center).

Western blot of 293 cells mock transfected (lane 1) or transiently transfected with pLenti6/TR lentiviral vector (lanes 2 & 3). Blots were probed with anti-Bsd (lanes 1-3). Molecular weight (MW) standards show that Bsd is expressed as a 13 kDa band in only the transfected cells.

Volume: 100 µL

Variant

Price:

$309

Quantity:

Bulk Order Anti-Blasticidin S Deaminase Antibody

Product Details

Target

Blasticidin S Deaminase

Target Description

Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week. Catalog# BP1231 is a Bsd antibody that can detect Bsd expression in cells transfected with blasticidin-selectable constructs. This antibody is useful for detection of successful gene transfer in constructs that utilize blasticidin selection.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

BSD

Molecular Weight

Antigen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase* from Aspergillus Terreus.

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 13 kDa* protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

BSD, blasticidin

UniProt Details

UniProt (Immunogen Species): P0C2P0

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Blasticidin S Deaminase Antibody