Anti-β-Catenin (Tyr-86), Phosphospecific Antibody

Name: Anti-β-Catenin (Tyr-86), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CP1191
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

CTNNB1

Molecular Weight

Go to Full Product Details

Immunocytochemical labeling of β-Catenin in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal β-Catenin (CM1181) or rabbit polyclonal β-Catenin (Tyr-86) or β-Catenin (Y654) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot of β-Catenin immunoprecipitated from A431 cells treated with pervanadate. The blots were probed with β-Catenin (Tyr-86) or β-Catenin (a.a. 649-661) antibodies. The immunoprecipitates were untreated (lanes 1 & 6) or treated with alkaline phosphatase (lanes 5 & 10), and the antibodies were incubated with β-Catenin (Tyr-86) (lanes 2,7) or β-Catenin (a.a. 649-661) (lanes 3,8) peptides or phospho-Tyr (lanes 4,9).

Volume: 100 µL

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$309

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Bulk Order Anti-β-Catenin (Tyr-86), Phosphospecific Antibody

Product Details

Target

β-Catenin (Tyr-86)

Target Description

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

CTNNB1

Molecular Weight

Antigen

Phospho-β-Catenin (Tyr-86) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 86 of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho- β-Catenin (Tyr-86) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells treated with pervanadate (1 mM for 30 min), but not in control cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-86

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Catenin beta1, CTNNB1, catenin

UniProt Details

UniProt (Human): P35222
UniProt (Immunogen Species): P35222

Product Specific Protocols

Western Blot Protocol: Download

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Anti-β-Catenin (Tyr-86), Phosphospecific Antibody