Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-β-Catenin (CM1181) (lanes 1 & 2), anti-β-Catenin (Tyr-654) (CP4021) (lanes 3 & 4), or anti-γ-Catenin (CM1111) (lanes 5 & 6).
Bulk Order Anti-β-Catenin (Tyr-654) [γ-Catenin (Tyr-644)], Phosphospecific Antibody
β-Catenin (Tyr-654)[γ-Catenin (Tyr-644)]
β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.
Antigen Affinity Purified
Phospho-β-Catenin (Tyr-654) synthetic peptide corresponds to amino acid residues around tyrosine 654 of human β-Catenin. This peptide sequence is highly conserved in rat, mouse, chicken, and frog β-Catenins, and has high homology to the conserved site in γ-Catenin (Tyr-644).
Chicken, Frog, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
This antibody was cross-adsorbed to phospho-β-Catenin (Tyr-86) peptide before affinity purification using phospho-β-Catenin (Tyr-654) peptide (without carrier). The antibody detects 84 and 88kDa* proteins corresponding to the molecular mass of γ-Catenin and β-Catenin, respectively, on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but does not detect these proteins in control cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.