Anti-β-Catenin Antibody (M118)

Name: Anti-β-Catenin Antibody
Brand: PhosphoSolutions
SKU: CM1181
Price: 340.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M118

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Format

Protein A Purified

Gene Name

CTNNB1

Molecular Weight

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Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-β-Catenin (CM1181) then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489) conserved site (CP2961), or anti-β-Catenin (CM1181).

Volume: 100 µL

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Product Details

Target

β-Catenin

Target Description

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M118

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

CTNNB1

Molecular Weight

Antigen

Clone (M118) was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:250
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 92 kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells. In addition, this antibody recognizes only β-Catenin in immunoprecipitations using anti-β-Catenin versus γ-Catenin.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Catenin beta1, CTNNB1, catenin

UniProt Details

UniProt (Human): P35222
UniProt (Immunogen Species): P35222

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Anti-β-Catenin Antibody (M118)

Anti-β-Catenin Antibody
SKU: CM1181

Bulk Order Anti-β-Catenin Antibody

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