Anti-Arpc1b/p41-Arc (Thr-21), Phosphospecific Antibody

Name: Anti-Arpc1b/p41-Arc (Thr-21), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: AP4351
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

2 Citations

Product Type

Primary Antibody

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

ARPC1B

Molecular Weight

Go to Full Product Details

Western blot analysis of Arpc1b phosphorylation in human A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).

Volume: 100 µL

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$309

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Product Details

Target

Arpc1b/p41-Arc (Thr-21)

Target Description

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. p41-Arc (Arpc1b) subunit Arpc1 has two isoforms in humans, Arpc1a and Arpc1b. PAK1 can bind and phosphorylate Thr-21 in Arpc1b leading to growth factor-stimulated cell motility. In addition, Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on Thr-21 and a nonphosphorylatable Arpc1b mutant cannot activate Aurora A kinase and centrosome amplification. Thus, Arpc1b has roles in cytoskeletal dynamics during cell motility and mitosis, and these activities are regulated by phosphorylation at Thr-21

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

ARPC1B

Molecular Weight

Antigen

Phospho-Arpc1b/p41-Arc (Thr-21) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding threonine 21 in human Arpc1b. This peptide sequence is highly conserved in rat, mouse, chicken, and fish Arpc1b, and has some homology to the conserved site in isoform Arpc1a.

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

The antibody detects a 41 kDa* protein corresponding to the molecular mass of Arpc1b on SDS-PAGE immunoblots of human A431 and mouse C2C12 treated with calyculin A. This reactivity is removed with lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr-21

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Arc41, p41, Arpc1b

UniProt Details

UniProt (Human): O15143
UniProt (Immunogen Species): O15143

Product Specific References for Applications and Species

Western Blot: Human | Rat

Western Blot: Human
PMID	Dilution	Publication
26160174	not listed	Kim, YB, et al. 2015. The Role of the Pleckstrin Homology Domain-containing Protein CKIP-1 in Activation of p21-activated Kinase 1 (PAK1). The Journal of Biological chemistry, 21076-21085.

Western Blot: Rat
PMID	Dilution	Publication
28972183	not listed	Tunduguru, R, et al. 2017. The actin-related p41ARC subunit contributes to p21-activated kinase-1 (PAK1)-mediated glucose uptake into skeletal muscle cells. The Journal of biological chemistry, 19034-19043.

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Anti-Arpc1b/p41-Arc (Thr-21), Phosphospecific Antibody