Anti-Argonaute 2 (N-terminus) Antibody (11A9)

Name: Anti-Argonaute 2 (N-terminus) Antibody
Brand: PhosphoSolutions
SKU: AM5271
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Bovine, Human

Clone

11A9

Applications

Host Species

Rat

Format

Protein G Purified

Gene Name

AGO2

Molecular Weight

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Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2 (AM5271) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).

Volume: 100 µL

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Product Details

Target

Argonaute 2 (N-terminus)

Target Description

Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.

Format

Protein G Purified

Clonality

Monoclonal

Clone

11A9

Isotype

IgG2a

Applications

Host Species

Rat

Gene Name

AGO2

Molecular Weight

Antigen

Clone 11A9 was generated from an Ago2 synthetic peptide (coupled to KLH) corresponding to amino acids in the N-terminus of human Ago2. This sequence has low homology to other argonaute family members.

Species Reactivity

Bovine, Human

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.05% NaN3

Dilution Ranges

WB: 1:250

Conjugate

Unconjugated

Specificity

The antibody detects a 97 kDa* protein corresponding to the apparent molecular mass of Ago2 on SDS-PAGE immunoblots of several human cell lines, A431, HeLa, MCF-7, and K562. The antibody does not detect rat or mouse Ago2, but will detect bovine Ago2.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Ago2, Argonaute, eIF-2C2, eIF2C2; protein slicer

UniProt Details

UniProt (Human): Q9UKV8
UniProt (Immunogen Species): Q9UKV8

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Anti-Argonaute 2 (N-terminus) Antibody (11A9)

Anti-Argonaute 2 (N-terminus) Antibody
SKU: AM5271

Bulk Order Anti-Argonaute 2 (N-terminus) Antibody

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Anti-Argonaute 2 (N-terminus) Antibody (11A9)

Anti-Argonaute 2 (N-terminus) Antibody SKU: AM5271

Bulk Order Anti-Argonaute 2 (N-terminus) Antibody

Product Details

Anti-Argonaute 2 (N-terminus) Antibody
SKU: AM5271