Anti-Annexin A1 Antibody (M021)

Name: Anti-Annexin A1 Antibody
Brand: PhosphoSolutions
SKU: AM0211
Price: 340.0 USD
Availability: InStock

from PhosphoSolutions

2 Citations

Product Type

Primary Antibody

Species Reactivity

Human

Clone

M021

Applications

ICC, IP, WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

ANXA1

Molecular Weight

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Immunocytochemical labeling of Annexin A1 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-Annexin A1 (clone M021). The antibody was detected using goat anti-mouse Ig DyLight® 594.

Western blot of human A431 cells (lane 1), A549 cells (lane 2), LNCaP cells (lane 3), full-length recombinant human annexin A1 (lane 4), and recombinant human annexin A2 (lane 5). The blot was probed with mouse monoclonal anti-Annexin A1 antibody (AM0211) at 1:1000 (lanes 1-5).

Volume: 100 µL

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Price:

$340

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Product Details

Target

Annexin A1

Target Description

The Annexin family is composed of at least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. Annexins have roles in membrane fusion, endocytosis, secretion, and repair. Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M021

Isotype

IgG1

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

ANXA1

Molecular Weight

Antigen

Clone M021 was generated from a proprietary antigen related to human Annexin A1 in the A549 lung carcinoma cell line.

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

Clone M021 detects a 38 kDa* protein corresponding to the molecular mass of Annexin A1 on SDS-PAGE immunoblots of human A431, MDA-MB-231, and A549. The antibody also detects full length recombinant human Annexin A1, but not Annexin A2 or Annexin A5 recombinant proteins. The antibody works for western blot, ELISA, immunocytochemical labeling, and immunoprecipitation.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I1, Phospholipase A2 inhibitory protein p35, ANXA1, ANX1, LPC1

UniProt Details

UniProt (Human): P04083
UniProt (Immunogen Species): P04083

Product Specific References for Applications and Species

ELISA: Human
Immunocytochemistry: Human
Immunoprecipitation: Human
Western Blot: Human

ELISA: Human
PMID	Dilution	Publication
32420875	1:1000	Vais, H, et al. 2020. ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein. eLife, e53531.

Immunocytochemistry: Human
PMID	Dilution	Publication
32420875	1:100	Vais, H, et al. 2020. ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein. eLife, e53531.

Immunoprecipitation: Human
PMID	Dilution	Publication
32420875	1:100	Vais, H, et al. 2020. ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein. eLife, e53531.

Western Blot: Human
PMID	Dilution	Publication
32420875	1:1000	Vais, H, et al. 2020. ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein. eLife, e53531.
30057678	not listed	Vaňková, K, et al. 2018. Chlorophyll-Mediated Changes in the Redox Status of Pancreatic Cancer Cells Are Associated with Its Anticancer Effects. Oxidative medicine and cellular longevity, 4069167.

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Anti-Annexin A1 Antibody (M021)

Anti-Annexin A1 Antibody
SKU: AM0211

Bulk Order Anti-Annexin A1 Antibody

Product Details

Product Specific References for Applications and Species

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Anti-Annexin A1 Antibody (M021)

Anti-Annexin A1 Antibody SKU: AM0211

Bulk Order Anti-Annexin A1 Antibody

Product Details

Product Specific References for Applications and Species

Anti-Annexin A1 Antibody
SKU: AM0211