Our Anti-Androgen Receptor (Ser94) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Androgen Receptor (Ser94) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of HeLa lysate showing specific immunolabeling of the ~110 kDa AR protein phosphorylated at Ser94 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).
The androgen receptor (AR) is a DNA-binding transcription factor that regulates genes critical for the development and maintenance of the male sexual phenotype. Defects in androgen receptor have been shown to play a role in prostate cancer, and inhibition of AR activity through modulation of signal transduction pathways may delay prostate cancer progression (Heinlein and Chang 2004). Multiple phosphorylation sites have been identified on the androgen receptor that affect cross-talk between growth factor signaling and androgen in prostate development and cancer (Gioeli et al., 2002). One of these sites, at Ser94, appears constitutively phosphorylated and exhibits no response to treatments with stimulating hormone (Gioeli et al., 2002). The site at Ser94 is unique among the AR phosphorylation sites in that it does not achieve a maximal level of phosphorylation between translation and the initial round of nuclear import, having a strong bias for androgen-independent phosphorylation in the cytoplasm (Kesler et al., 2007).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
AR
110 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser94 of human androgen receptor, conjugated to keyhole limpet hemocyanin (KLH).
Human
Non-Human Primate
AB_2492040
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~110k AR protein phosphorylated at Ser94. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to generate the antibody but not by the corresponding dephospho-peptide.
Phosphorylated
Ser94
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Heinlein C, Chang C. (2004) Androgen Receptor in Prostate Cancer.Endocrine Reviews. 25(2):276-308. PMID: 15082523
Gioeli D, Ficarro S, Kwiek J, Aaronson D, Hancock M, Catling A, White F, Christian R, Settlage R, Shabanowitz J, Hunt D, Weber M. (2002) Androgen Receptor Phosphorylation, Regulation and Indentification of the Phosphorylation Sites. J Biol Chem. 277(32):29304-29314. PMID: 12015328
Kesler C, Gioeli D, Conaway M, Weber M, Paschal B. (2007)Subcellular Localization Modulates Activation Function 1 Domain Phosphorylation in the Androgen Receptor.Molecular Endocrinology. 21(9):2071-2084. PMID: 17579212
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