Immunofluorescence of cultured neurons and glia cells showing specific labeling of neuronal perikarya, axons and dendrites with anti-alpha-II-spectrin (cat. 99-A2SR, 1:1000, green), specific labeling of intermediate filaments in glial and fibroblastic cells with anti-vimentin (cat. 2105-VIM, 1:5000, red) and nuclear staining with DAPI (blue).
Anti-Alpha II Spectrin Antibody
Alpha II Spectrin
The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (α1 and α2) and five beta genes (β1-β5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004).
ICC, IHC, WB
Recombinant construct containing the 7th, 8th, 9th repeats of human alpha II spectrin.
Human, Mouse, Rat
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.