Name: Anti-Alpha II Spectrin Antibody
Brand: PhosphoSolutions
SKU: 99-A2SR
Price: 360.0 USD
Availability: InStock

Our Anti-Alpha II Spectrin primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat Alpha II Spectrin and is serum. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

Format

Serum

Gene Name

SPTAN1

Molecular Weight

240 kDa

Go to Full Product Details

Western blot of mouse brain lysate showing specific immunolabeling of the ~240 kDa alpha II spectrin protein.

Immunofluorescence of cultured neurons and glia cells showing specific labeling of neuronal perikarya, axons and dendrites with anti-alpha-II-spectrin (cat. 99-A2SR, 1:1000, green), specific labeling of intermediate filaments in glial and fibroblastic cells with anti-vimentin (cat. 2105-VIM, 1:5000, red) and nuclear staining with DAPI (blue).

Volume: 100 µL

Variant

Price:

$360

Quantity:

Bulk Order Anti-Alpha II Spectrin Antibody

Product Details

Target

Alpha II Spectrin

Target Description

The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (α1 and α2) and five beta genes (β1-β5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004).

Format

Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

SPTAN1

Molecular Weight

240 kDa

Antigen

Recombinant construct containing the 7th, 8th, 9th repeats of human alpha II spectrin.

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492031

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Neat serum

Buffer

Neat serum

Dilution Ranges

WB: 1:5000
WB Brain: 1:5000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~240 kDa alpha II spectrin protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

(ALPHA)II-SPECTRIN antibody, Alpha-II spectrin antibody, brain antibody, EIEE5 antibody, FLJ17738 antibody, FLJ44613 antibody, Fodrin antibody, Fodrin alpha chain antibody, Fodrin, alpha antibody, NEAS antibody, Non erythrocytic spectrin alpha antibody, non-erythroid alpha chain antibody,SPECA antibody, Spectrin alpha chain antibody, Spectrin alpha chain brain antibody, Spectrin alpha non erythrocytic 1 antibody, Spectrin antibody, Spectrin non erythroid alpha chain antibody, Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody, Spectrin, nonerythroid, alpha subunit antibody, Spna2 antibody, SPTA 2 antibody, SPTA2 antibody, SPTA2_HUMAN antibody, SPTAN 1 antibody, SPTAN1 antibody

UniProt Details

UniProt (Human): Q13813
UniProt (Immunogen Species): Q13813

NCBI Gene ID

6709

Product Specific Protocols

Western Blot Protocol: Download

General References

Bennett, V. and Baines, A.J., 2001. Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues. Physiological Reviews, 81(3), pp.1353-1392. PMID: 15882777
Eber, S. and Lux, S.E., 2004, April. Hereditary spherocytosis—defects in proteins that connect the membrane skeleton to the lipid bilayer. In Seminars in Hematology, 41(2),pp. 118-141. PMID: 15882777

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Anti-Alpha II Spectrin Antibody

Our Anti-Alpha II Spectrin primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat Alpha II Spectrin and is serum. It is great for use in WB, IHC, ICC.