Name: Anti-Alpha Actinin 4 Antibody
Brand: PhosphoSolutions
SKU: 127-ACTN4
Price: 415.0 USD
Availability: InStock

Our Anti-Alpha Actinin 4 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human and mouse Alpha Actinin 4 and is Protein G purified. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse

Clone

93

Applications

IHC, WB

Host Species

Mouse

Format

Protein G purified

Gene Name

ACTN4

Molecular Weight

105 kDa

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Western blot of mouse whole brain lysate showing specific immunolabeling of the ~105 kDa α-actinin 4 protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Alpha Actinin 4 Antibody

Product Details

Target

Alpha Actinin 4

Target Description

α-actinin-4 is a member of the actinin protein family comprised of an actin-binding domain in the N-terminus, 4 spectrin-like repeats in the central region, and 2 EF-hand motifs in the C-terminus (Honda et al, 1998). α-actinin-4 and CLP36 form a complex in normal kidney podocytes.CLP36 is dependent on α-actinin-4 for maintenance of its level in podocytes, whereas α-actinin-4 is independent of CLP36. α-actinin-4 is widely expressed in mammalian tissues and organs, while having a high occurrence of genetic mutations in kidney podocytes (Kos et al, 2003). FSGS, focal segmental glomerulosclerosis, is a rare genetic disease that attacks the kidney's filtering units (glomeruli) causing serious scarring which leads to permanent kidney damage and even failure. Three key mutations have been found in α-actinin-4 in people diagnosed with FSGS. R310Q and Q348R, located in the spectrin-like repeats region, and K255E located in the actin-binding region. The R310Q and Q348R mutation significantly inhibits the ability of α-actinin-4 to form the complex with CLP36. The K255E mutation was reversed where it increased the ability to bind CLP36 in the actin-binding region (Liu et al, 2011).

Format

Protein G purified

Clonality

Monoclonal

Clone

93

Isotype

IgG

Applications

IHC, WB

Host Species

Mouse

Gene Name

ACTN4

Molecular Weight

105 kDa

Antigen

Fusion protein from the central rod domain of human α-actinin 4.

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_2736847

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified cultured supernatant.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:250

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~105 kDa α-actinin 4 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

actinin 4 antibody, Actinin alpha 4 antibody, actinin4 antibody, ACTN 4 antibody, ACTN4 antibody, ACTN4_HUMAN antibody, alpha Actinin 4 antibody, Alpha-actinin-4 antibody, DKFZp686K23158 antibody, F actin cross linking protein antibody, F-actin cross-linking protein antibody, Focal segmental glomerulosclerosis 1 antibody, FSGS 1 antibody, FSGS antibody, FSGS1 antibody, Non muscle alpha actinin 4 antibody, Non-muscle alpha-actinin 4 antibody

UniProt Details

UniProt (Immunogen Species): O43707

Product Specific References for Applications and Species

Immunohistochemistry: Human
Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
21680739	not listed	Liu, Z., et al. 2011α-Actinin-4 and CLP36 protein deficiencies contribute to podocyte defects in multiple human glomerulopathies. Journal of Biological Chemistry, 286(35), pp.30795-30805.

Western Blot: Human
PMID	Dilution	Publication
21680739	not listed	Liu, Z., et al. 2011α-Actinin-4 and CLP36 protein deficiencies contribute to podocyte defects in multiple human glomerulopathies. Journal of Biological Chemistry, 286(35), pp.30795-30805.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Alpha Actinin 4 Antibody (93)

Our Anti-Alpha Actinin 4 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human and mouse Alpha Actinin 4 and is Protein G purified. It is great for use in WB, IHC.

Anti-Alpha Actinin 4 Antibody
SKU: 127-ACTN4

Bulk Order Anti-Alpha Actinin 4 Antibody

Product Details

Product Specific References for Applications and Species

Product Specific Protocols

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Anti-Alpha Actinin 4 Antibody (93)

Our Anti-Alpha Actinin 4 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human and mouse Alpha Actinin 4 and is Protein G purified. It is great for use in WB, IHC.

Anti-Alpha Actinin 4 Antibody SKU: 127-ACTN4

Bulk Order Anti-Alpha Actinin 4 Antibody

Product Details

Product Specific References for Applications and Species

Product Specific Protocols

Anti-Alpha Actinin 4 Antibody
SKU: 127-ACTN4