Western blot of mouse whole brain lysate showing specific immunolabeling of the ~105 kDa α-actinin 4 protein.
Anti-Alpha Actinin 4 Antibody
Alpha Actinin 4
α-actinin-4 is a member of the actinin protein family comprised of an actin-binding domain in the N-terminus, 4 spectrin-like repeats in the central region, and 2 EF-hand motifs in the C-terminus (Honda et al, 1998). α-actinin-4 and CLP36 form a complex in normal kidney podocytes.CLP36 is dependent on α-actinin-4 for maintenance of its level in podocytes, whereas α-actinin-4 is independent of CLP36. α-actinin-4 is widely expressed in mammalian tissues and organs, while having a high occurrence of genetic mutations in kidney podocytes (Kos et al, 2003). FSGS, focal segmental glomerulosclerosis, is a rare genetic disease that attacks the kidney's filtering units (glomeruli) causing serious scarring which leads to permanent kidney damage and even failure. Three key mutations have been found in α-actinin-4 in people diagnosed with FSGS. R310Q and Q348R, located in the spectrin-like repeats region, and K255E located in the actin-binding region. The R310Q and Q348R mutation significantly inhibits the ability of α-actinin-4 to form the complex with CLP36. The K255E mutation was reversed where it increased the ability to bind CLP36 in the actin-binding region (Liu et al, 2011).
Protein G purified
Fusion protein from the central rod domain of human α-actinin 4.
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Protein G purified cultured supernatant.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB Brain: 1:1000
Specific for endogenous levels of the ~105 kDa α-actinin 4 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.