Anti-Akt (Ser-473), Phosphospecific Antibody (M114)

Name: Anti-Akt (Ser-473), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: AM1141
Price: 329.0 USD
Availability: InStock

3 Citations

Our Akt (Ser-473) phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Akt (Ser-473) and is Protein G purified. It is great for use in WB, IP.

Western blot analysis of A431 cells untreated (lanes 1 & 3) or treated with 100 ng/ml EGF for 60 min. (lanes 2 & 4). The blots were probed with monoclonal anti-phospho-Akt (Ser-473) (lanes 1 & 2) and monoclonal anti-Akt1 (N-terminal region) (lanes 3 &4).

Species Reactivity

Human, Mouse, Rat

Applications

IP, WB

Host Species

Mouse

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Data Sheet

SKU: AM1141

Product Details

Target

Akt (Ser-473)

Target Description

Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M114

Isotype

IgG1

Applications

IP, WB

Host Species

Mouse

Gene Name

AKT1

Molecular Weight

Antigen

Clone M114 was generated from a peptide containing amino acid residues surrounding Serine 473 in human Akt1. This sequence is highly conserved in human and mouse Akt, and may recognize Akt2 and Akt3.

Species Reactivity

Human, Mouse, Rat

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.02% NaN3

Dilution Ranges

WB: 1:250
IP: 1:100

Conjugate

Unconjugated

Specificity

This antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of Akt on SDS-PAGE immunoblots of mouse NIH3T3 cells treated with PDGF and human A431 cells treated with EGF.

Post Translational Modification Type

Phosphorylated

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PKBalpha, PKB, AKT

UniProt Details

UniProt (Human): P31749
UniProt (Immunogen Species): P31749

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat

Western Blot: Human
PMID	Dilution	Publication
30941654	not listed	Hu, C, et al. 2019. Resveratrol inhibits the proliferation of estrogen receptor-positive breast cancer cells by suppressing EZH2 through the modulation of ERK1/2 signaling. Cell biology and toxicology, 445-456.

Western Blot: Mouse
PMID	Dilution	Publication
21496122	1:250	Paterniti, I, et al. 2011. Evidence for the role of PI3-kinase-AKT-eNOS signalling pathway in secondary inflammatory process after spinal cord compression injury in mice. The European Journal of Neuroscience, 1411-1420.

Western Blot: Rat
PMID	Dilution	Publication
38004105	1:1000	Rodrigues Junior, CF, et al. 2023. Changes in Skeletal Muscle Protein Metabolism Signaling Induced by Glutamine Supplementation and Exercise. Nutrients, .

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Anti-Akt (Ser-473), Phosphospecific Antibody (M114)

SKU: AM1141

Product Details

Product Specific References for Applications and Species

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