Anti-AIFM1 (Ser-116), Phosphospecific Antibody

Name: Anti-AIFM1 (Ser-116), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: AP5501
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

8 Citations

Product Type

Primary Antibody

Species Reactivity

Human

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

AIFM1

Molecular Weight

57/62/66

Go to Full Product Details

Western blotting shows KEAP1-PGAM5-AIFM1 pathway protein level regulation by ALT. SKOV-3 cells were treated with different concentrations (0, 16, 32 and 64 μM) of ALT for 24 h. Specifically AIFM1, P-AIFM1(Ser-116) (cat. AP5501), KEAP1, Nrf2, and PGAM5 levels were detected. B-Actin used as a control. Image from publication CC-BY-4.0. PMID: 37712005

Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIFM1 (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIFM1 (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).

Volume: 100 µL

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Product Details

Target

AIFM1 (Ser-116)

Target Description

Apoptosis-inducing factor (AIFM1, AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis. AIFM1 is expressed as a 66 kDa precursor protein before being N-terminally cleaved to 62 kDa and localized to the mitochondrial intermembrane space. In response to apoptotic stimuli, AIFM1 is released from the mitochondrial intermembrane as a 57 kDa fragment that can translocate to the nucleus. Treatment of isolated nuclei with recombinant AIFM1 leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation. Studies of AIFM1 knockout mice have shown that the apoptotic activity of AIFM1 is cell type and stimuli-dependent. AIFM1 has been implicated in oxeiptosis, a non-inflammatory, caspase independent cell death pathway caused by oxidative stress. During oxeiptosis, increased reactive oxygen species cause the release of the phosphatase PGAM5 from KEAP1 leading to dephosphorylation of AIFM1 (Ser-116) and subsequent cell death. Thus, AIFM1 phosphorylation status at Ser-116 may be an important marker for cell death involving oxeiptosis.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

AIFM1

Molecular Weight

57/62/66

Antigen

Phospho-AIFM1 (Ser-116) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-116 in human AIFM1. This site is conserved in rat and mouse AIFM1, but the amino acids surrounding the site are not well conserved. The site is not found in AIFM2 and AIFM3.

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody was cross adsorbed to unphosphorylated AIFM1 (Ser-116) peptide before affinity purification using phospho-AIFM1 (Ser-116) peptide. This antibody antibody detects a triplet at 66, 62, and 57 kDa* protein on SDS-PAGE immunoblots of human Jurkat cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment to dephosphorylated AIFM1.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-116

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PDCD8, AIF1, Apoptosis inducing factor 1, mitochondrial; Program cell death protein 8; striatal AIF

UniProt Details

UniProt (Human): O95831
UniProt (Immunogen Species): O95831

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Human
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
29255269	not listed	Holze, C, et al. 2018. Oxeiptosis, a ROS-induced caspase-independent apoptosis-like cell-death pathway. Nature immunology, 130-140.

Immunohistochemistry: Human
PMID	Dilution	Publication
37741130	1:200	You, M, et al. 2023. 4-Octyl itaconate inhibits inflammation to attenuate psoriasis as an agonist of oxeiptosis. International Immunopharmacology, 110915.

Western Blot: Human
PMID	Dilution	Publication
37741130	not listed	You, M, et al. 2023. 4-Octyl itaconate inhibits inflammation to attenuate psoriasis as an agonist of oxeiptosis. International immunopharmacology, 110915.
37712005	not listed	Nasirzadeh, M, et al. 2023. Alantolactone triggers oxeiptosis in human ovarian cancer cells via Nrf2 signaling pathway. Biochemistry and Biophysics Reports, 101537.
36626243	not listed	Tsui, KH, et al. 2023. Mitoquinone shifts energy metabolism to reduce ROS-induced oxeiptosis in female granulosa cells and mouse oocytes. Aging, 246-260.
35177586	1:1000	Kang, P, et al. 2022. Oxeiptosis: a novel pathway of melanocytes death in response to oxidative stress in vitiligo. Cell death discovery, 70.
34872005	1:1000	Wang, CX, et al. 2022. Auriculasin enhances ROS generation to regulate colorectal cancer cell apoptosis, ferroptosis, oxeiptosis, invasion and colony formation. Biochemical and Biophysical Research Communications, 99-106.

Western Blot: Mouse
PMID	Dilution	Publication
36914635	not listed	Pallichankandy, S, et al. 2023. Targeting oxeiptosis-mediated tumor suppression: a novel approach to treat colorectal cancers by sanguinarine. Cell death discovery, 94.

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Anti-AIFM1 (Ser-116), Phosphospecific Antibody