Anti-AIF1/Iba1 Antibody

Name: Anti-AIF1/Iba1 Antibody
Brand: PhosphoSolutions
SKU: 91-AIF1
Price: 415.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Trial Size

Pooled Serum

Our Anti-AIF1/Iba1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse AIF1/Iba1 and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse

Expected Reactivity

Human

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

AIF1

Molecular Weight

17 kDa

Go to Full Product Details

Western blot of mouse spleen lysate showing specific immunolabeling of the ~17 kDa AIF1/Iba1 protein.

Immunofluoresence of FLAG-tagged AIF1 transfected COS cells showing AIF1 staining in red (cat. 91-AIF1, 1:50) and FLAG staining in green causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-AIF1/Iba1 Antibody

Product Details

Target

AIF1/Iba1

Target Description

AIF1, allograft inflammatory factor-1, (also known as Iba1, Ionized calcium binding adaptor molecule 1) is a 17 kDa protein that is a pro-inflammatory molecule that mediates calcium signals (Ito, D. et al 1998). During inflammation, increased expression of AIF1 occurs in microglia, macrophages, T-cells, synoviocytes, and adipocytes (Watano, K., et al, 2001). Expression of AIF-1 has been observed in healthy brains and varies throughout the day, gaining the highest expression in the prefrontal and parietal cortices when sleeping (Romcy-Pereira, R.N, et al, 2009). Increased expression of AIF1 has been observed in several diseases including breast cancer (Liu, S., et al 2008), atherosclerosis (Utans, U., et al, 1995), and rheumatoid arthritis (Kimura, M., et al, 2007).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

AIF1

Molecular Weight

17 kDa

Antigen

Full-length human AIF1/Iba1 protein expressed in and purified from E. coli.

Species Reactivity

Human, Mouse

Expected Reactivity

Human

Antibody Registry ID

AB_2868532

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the protein immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
ICC: 1:500-1:5000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~17 kDa AIF1/Iba1 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

UniProt Details

UniProt (Human): P55008
UniProt (Immunogen Species): P55008

NCBI Gene ID

199

Product Specific Protocols

Western Blot Protocol: Download

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Anti-AIF1/Iba1 Antibody

Our Anti-AIF1/Iba1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse AIF1/Iba1 and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.