Our Anti-AIF1/Iba1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse AIF1/Iba1 and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.
Immunofluoresence of FLAG-tagged AIF1 transfected COS cells showing AIF1 staining in red (cat. 91-AIF1, 1:50) and FLAG staining in green causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.
AIF1, allograft inflammatory factor-1, (also known as Iba1, Ionized calcium binding adaptor molecule 1) is a 17 kDa protein that is a pro-inflammatory molecule that mediates calcium signals (Ito, D. et al 1998). During inflammation, increased expression of AIF1 occurs in microglia, macrophages, T-cells, synoviocytes, and adipocytes (Watano, K., et al, 2001). Expression of AIF-1 has been observed in healthy brains and varies throughout the day, gaining the highest expression in the prefrontal and parietal cortices when sleeping (Romcy-Pereira, R.N, et al, 2009). Increased expression of AIF1 has been observed in several diseases including breast cancer (Liu, S., et al 2008), atherosclerosis (Utans, U., et al, 1995), and rheumatoid arthritis (Kimura, M., et al, 2007).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, WB
Rabbit
AIF1
17 kDa
Full-length human AIF1/Iba1 protein expressed in and purified from E. coli.
Human, Mouse
Human
AB_2868532
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification using a column to which the protein immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
ICC: 1:500-1:5000
Unconjugated
Specific for endogenous levels of the ~17 kDa AIF1/Iba1 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
