Anti-A-Raf (N-terminal region) Antibody (M289)

Name: Anti-A-Raf (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: RM2891
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M289

Applications

Host Species

Mouse

Format

Protein G Purified

Gene Name

ARAF

Molecular Weight

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Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-A-Raf (N-terminal region) antibody at 1:500 (lane 1) and 1:2000 (lane 2).

Volume: 100 µL

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$255

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Product Details

Target

A-Raf (N-terminal region)

Target Description

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and Ser-471. The latter site is phosphorylated after EGF stimulation and may be important for MEK interaction in both C-Raf and A-Raf. In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain. Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation. Ser-579 is required for growth factor activation and kinase activity.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M289

Isotype

IgG1

Applications

Host Species

Mouse

Gene Name

ARAF

Molecular Weight

Antigen

Clone (M289) was generated from a sequence corresponding to amino acids in the N-terminal region of human A-Raf. This A-Raf sequence has high homology to rat and mouse A-Raf.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody detects a 68 kDa* protein corresponding to the molecular mass of A-Raf on SDS-PAGE immunoblots of human SKN-SH and Jurkat cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

araf

UniProt Details

UniProt (Human): P10398
UniProt (Immunogen Species): P10398

Product Specific Protocols

Western Blot Protocol: Download

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Anti-A-Raf (N-terminal region) Antibody (M289)

Anti-A-Raf (N-terminal region) Antibody
SKU: RM2891

Bulk Order Anti-A-Raf (N-terminal region) Antibody

Product Details

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Anti-A-Raf (N-terminal region) Antibody (M289)

Anti-A-Raf (N-terminal region) Antibody SKU: RM2891

Bulk Order Anti-A-Raf (N-terminal region) Antibody

Product Details

Product Specific Protocols

Anti-A-Raf (N-terminal region) Antibody
SKU: RM2891