NCC Thr53 Antibody
The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015). Phosphorylation of NCC at Thr-53, Thr-58, and Ser-71 is an essential mediator of NCC function (Rosenbaek et al., 2014). NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015).
Made right here at PhosphoSolutions as a custom antibody, and released to the public due to high demand, our NCC thr53 is phosphospecific and reliable. Referenced in multiple publications, and validated in knockouts, you won’t regret giving our antibody a try!
||Rabbit Polyclonal, Affinity Purified||100 ul||p1311-53||$365.00|
Product Specific References
- Tutakhel, O.A., Bianchi, F., Smits, D.A., Bindels, R.J., Hoenderop, J.G. and van der Wijst, J., 2018. Dominant functional role of the novel phosphorylation site S811 in the human renal NaCl cotransporter. The FASEB Journal, pp.fj-201701047R. PMID: 29547703
- Palygin, O., Levchenko, V., Ilatovskaya, D.V., Pavlov, T.S., Pochynyuk, M., Jacob, H.J., Geurts, A.M., Hodges, M.R. and Staruschenko, A., 2017. Essential role of K ir 5.1 channels in renal salt handling and blood pressure control. JCI Insight, 2(18). PMID: 28931751
- Terker AS, Yarbrough B, Ferdaus MZ, Lazelle RA, Erspamer KJ, Meermeier NP, Park HJ, McCormick JA, Yang CL, Ellison D3. (2016) Direct and Indirect Mineralocorticoid Effects Determine Distal Salt Transport. J Am Soc Nephrol. (8):2436-45. PMID: 26712527
- Terker AS, Zhang C, McCormick JA, Lazelle RA, Zhang C, Meermeier NP, Siler D, Park HJ, Fu Y, Cohen DM, Weinstein AM, Wang WH, Yang CL, Ellison DH. (2015) Potassium modulates electrolyte balance and blood pressure through effects on distal cell voltage and chloride. Cell Metab. (1):39-50. PMID: 25565204
- McCormick, J. A., Nelson, J. H., Yang, C. L., Curry, J. N., & Ellison, D. H. (2011). Overexpression of the sodium chloride cotransporter is not sufficient to cause familial hyperkalemic hypertension. Hypertension, 58(5), 888-894. PMID: 21896937