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New Line of Cancer Antibodies

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Western blot analysis of Caspase-3 in multiple human tissues using CM4911 at 1:100. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis. Lanes 1, 2 and 3 demonstrate the species crossreactivity of the antibody in human, mouse and rat heart lysate, respectively.Induction of apoptosis after combination treatment was marked by poly (ADP-ribose) polymerase (PARP) cleavage and caspase-3 (cat. CM4911) activation. Western blots of single or combination treatment of LNCaP (48 h), DU145, PC-3 or CHO cells (all 72 h) with 1.0 µg/ml SO1861 and 2.5 nM EGF-PE24mutΔREDLK. β-actin was used as a loading control. *CHO cells are not of human origin - detection of human Caspase-3 was not possible in this cell line. Image from publication CC-BY-4.0. PMID: 37859824
Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 antibodies, and detected using appropriate secondary antibodies conjugated to Cy3. Phase contrast images (left) and  immunofluorescent images (right).
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M007). The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M010). The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M024). The antibody was detected using goat anti-mouse DyLight® 594.Representative Standard Curve using mouse monoclonal anti-CD44 (CM0241) for ELISA capture of human recombinant CD44 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Western blot of native lysates from NCI-H2052 mesothelial cells (lane 1), SK-MES-1 squamous carcinoma (lane 2), MDA-MB-231 breast carcinoma (lane 3), MeWo melanoma (lane 4), A431 skin adenocarcinoma (lane 5), LNCaP prostate cancer cells (lane 6), and MCF7 breast cancer cells (lane 7). The blot was probed with mouse monoclonal anti-CD46 (CM0371) at 1:1000.Immunocytochemical labeling of CD46 in aldehyde fixed human NCIH1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD46 (CM0371). The antibody was detected using goat anti-mouse Ig:DyLight® 594.
Native western blot of human BEAS-2B lung cells (lane 1), NCI-H446 lung cancer cells (lane 2), lung mesothelioma cells: NCI-H28 (lane 3), and NCI-H2052 (lane 4). The blot was probed with mouse monoclonal anti-CD47 (CM0541) at 1:500.Immunocytochemical labeling of CD47 in aldehyde fixed human MCF7 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-CD47 (CM0541). The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of CD55 in paraformaldehyde fixed human MDA-MB-231 breast cancer cells. The cells were labeled with mouse monoclonal anti-CD55 (CM0021). The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of CD55 in paraformaldehyde fixed human MDA-MB-231 breast cancer cells. The cells were labeled with mouse monoclonal anti-CD55 (CM0331). The antibody was detected using goat anti-mouse DyLight® 594.Representative Standard Curve using mouse monoclonal anti-CD55 (CM0331) for ELISA capture of human recombinant CD55
extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Western blot analysis of recombinant human CD59 protein (lanes 1 & 5), A431 (lanes 2 & 6), A549 (lanes 3 & 7), and MCF7 (lanes 4 & 8) whole cell lysates under native conditions. The blots were probed with mouse monoclonal anti-CD59 (CM0111) at 1:1000 (lanes 1-4) and anti-CD59 (CM0151) at 1:1000 (lanes 5-8).Immunocytochemical labeling of CD59 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-CD59 (clone M011). The antibody was detected using goat anti-mouse Ig DyLight® 594.

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